天津新手RNA提取試劑盒500T/5865元歡迎來(lái)電上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="向勻漿樣品中加0.2ml氯仿，蓋好管蓋，劇烈振蕩15秒，室溫放置3-5分鐘。2-8℃12000rpm離心10分鐘。RNA主要在上層無(wú)色的水相中，把水相轉(zhuǎn)移到新管中，不要吸到沉淀。吸附柱前處理：在吸附柱中加入500ul洗柱液，室溫放置2分鐘，"><meta name="keywords" content="天津新手RNA提取試劑盒500T/5865元,RNA提取試劑盒"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" 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href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項(xiàng)目合作</a>?天津新手RNA提取試劑盒500T/5865元歡迎來(lái)電上海朝瑞生物科技供應(yīng)</p><div id="4r84hv3" class="leftbox clearfix"><h1>天津新手RNA提取試劑盒500T/5865元歡迎來(lái)電上海朝瑞生物科技供應(yīng)</h1><div id="dtkwjaj" class="proBig"><div id="7zo3cdp" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739418232422/5654310.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="ptub3gn" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739418232422/5654310.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="nqhjq8u" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津新手RNA提取試劑盒500T/5865元,RNA提取試劑盒</p><p><span>***更新：</span>2020-06-30 06:18:30</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="tseqsjb" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="v2uwdua" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="srs8ijv" class="box"><p><p> 向勻漿樣品中加0.2ml氯仿，蓋好管蓋，劇烈振蕩15秒，室溫放置3-5分鐘。 </p> <p> 2-8℃ 12000 rpm離心10分鐘。RNA主要在上層無(wú)色的水相中，把水相轉(zhuǎn)移到新管中，不要吸到沉淀。 </p> <p> 吸附柱前處理：在吸附柱中加入500ul 洗柱液，室溫放置2分鐘，2-8℃ 12,000 rpm離心2min，棄廢液。 </p> <p> 第4步收集的上清中加入200ul無(wú)水乙醇混勻，加入吸附柱靜置2分鐘，2-8℃ 12000rpm離心2min，棄廢液。 </p> <p> 向吸附柱中加入600ul漂洗液(使用前請(qǐng)先檢查是否已加入無(wú)水乙醇)，2-8℃ 12,000 rpm離心2min，棄廢液。 </p> <p> 向吸附柱中加入600ul漂洗液，2-8℃ 12,000 rpm離心2min，棄廢液，天津新手RNA提取試劑盒500T/5865元。芯片和試劑，天津新手RNA提取試劑盒500T/5865元，對(duì)操作人員技術(shù)要求低，天津新手RNA提取試劑盒500T/5865元，全程只需20分鐘，患者等待周期短，成本低。天津新手RNA提取試劑盒500T/5865元</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津新手RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739418232422/5654310.png"></div><p> 別名總RNA提取試劑盒英文名稱Total RNA Extraction Kit儲(chǔ)存條件2-8℃，避光，有效期1年單位盒 </p> <p> 貨號(hào)：R1200 </p> <p> 規(guī)格：50T/100T </p> <p> 產(chǎn)品內(nèi)容：試劑盒組成R1200-50R1200-100保存有效期裂解液50ml100ml2-8℃一年漂洗液15ml30mlRT一年洗柱液50ml100mlRT一年RNase free ddH2O15ml30mlRT一年RNase free吸附柱50個(gè)100個(gè)RT一年RNase free收集管(2ml)50個(gè)100個(gè)RT一年 </p> <p> <br /> 江蘇四步完成RNA提取試劑盒信賴推薦取新鮮或-70℃凍存100mg**加1ml裂解液，用**研磨杵或勻漿器勻漿處理。</p> 操作步驟：樣品處理： a. 植物組織：取新鮮或-70℃凍存100mg組織在液氮中研磨，把粉末加入到1ml裂解液中混勻。<div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津新手RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414501953/9962850.png"></div><p> RNA提取注意事項(xiàng)（5） </p> <p> 純化得到的RNA可能需要通過(guò)沉淀來(lái)濃縮，以滿足一些下游應(yīng)用的需要。醋酸銨(NH4OAc) 沉淀（加0.1體積的5 M 醋酸銨、2-2.5體積的無(wú)水乙醇，-20°C放置25分鐘以上）可以很好地回收RNA。如果需要定量回收低濃度的RNA（ng/ml），可以采用共沉淀（如linear acrylamide糖原glycogen,、酵母yeast RNA ）的方法。核酸助沉劑是linear acrylamide，當(dāng)RNA用于RT-PCR分析時(shí)，線性的丙烯酰胺和DNase處理的糖原都可以作為理想的共沉淀劑，因?yàn)樗鼈兌疾缓珼NA污染，糖原含量高會(huì)制抑PCR反應(yīng)。酵母RNA和未處理的糖原會(huì)給樣品帶來(lái)核酸污染，有可能影響RT-PCR的結(jié)果。沉淀后，注意避免RNA沉淀過(guò)分干燥，因?yàn)檫@可能導(dǎo)致很難重新溶解。 </p> <p> <br /> </p><p> 本實(shí)用新型公開了RNA提取試劑盒，涉及生物技術(shù)領(lǐng)域。本實(shí)用新型包括盒蓋和盒體，盒體為圓柱形結(jié)構(gòu)，盒體內(nèi)通過(guò)橫板分隔為試劑腔和容納腔，試劑腔內(nèi)設(shè)有試劑存放盒，試劑存放盒內(nèi)設(shè)有固定層，固定層上均勻開設(shè)有若干個(gè)與試劑瓶相配合對(duì)應(yīng)的容器孔，固定層上設(shè)置有與容器孔相配合對(duì)應(yīng)的標(biāo)簽槽，標(biāo)簽槽中設(shè)置標(biāo)簽，容納腔內(nèi)設(shè)有***抽盒和第二抽盒，***抽盒滑動(dòng)設(shè)置在盒體內(nèi)，第二抽盒滑動(dòng)設(shè)置在盒體內(nèi)，***抽盒為工具箱，第二抽盒為試劑箱，盒蓋一表面設(shè)有提手。本實(shí)用新型通過(guò)設(shè)計(jì)圓柱形結(jié)構(gòu)的盒體，盒體內(nèi)置傾斜的固定層和相對(duì)應(yīng)的標(biāo)簽槽以及提手結(jié)構(gòu)，解決了現(xiàn)有的試劑盒空間利用率不高，試劑管容易混淆，不易攜帶的問(wèn)題。 </p> <p> <br /> 所提取的RNA完整性好、純度高，可用于分子生物學(xué)后實(shí)驗(yàn)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津新手RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739416328125/8706890.png"></div><p> 本產(chǎn)品中含有苯酚，具0性和腐蝕性。如果吸入體內(nèi)、接觸皮膚、吞食等會(huì)引起中毒、灼傷及其他身體傷害。使用時(shí)應(yīng)穿戴防護(hù)服裝、手套、面罩等防護(hù)物。如果不小心接觸到眼睛時(shí)，應(yīng)立即用大量水沖洗然后前往醫(yī)院進(jìn)行***。 </p> <p> 請(qǐng)穿實(shí)驗(yàn)服并佩戴一次性手套進(jìn)行實(shí)驗(yàn)操作，避免 RNase 污染。 </p> <p> 使用 RNase-free 的塑料制品和***頭避免交叉污染；所有離心管及相關(guān)溶液都必須無(wú) RNA 酶污染。 </p> <p> 請(qǐng)自備氯仿、異丙醇（新開封或提取 RNA **）、75%乙醇（DEPC 處理水配制）、DEPC 和 DEPC 處理水。 </p> <p> 使用凍存的細(xì)胞或組織抽提總 RNA 的效果通常會(huì)比新鮮的細(xì)胞或組織差一些，因?yàn)閮龃孢^(guò)程中細(xì)胞或組織內(nèi)的 RNase 會(huì)釋放出來(lái)并剪切樣品。推薦：如果不能及時(shí)抽提 RNA，先加入適量 Trizol 試劑，裂解樣品然后再凍存，這樣 RNase 就不會(huì)降解樣品。離心收集細(xì)胞。每106動(dòng)物、植物和酵母細(xì)胞或每107細(xì)菌細(xì)胞加1ml裂解液混勻。江蘇四步完成RNA提取試劑盒信賴推薦</p><p style="text-indent:25px">取0.2-1ml新鮮血液加3倍體積紅細(xì)胞裂解液，混勻后室溫放置10分鐘。天津新手RNA提取試劑盒500T/5865元</p><p> SimplyP 總RNA提取試劑 </p> <p> <br /> </p> <p> 把總RNA提取速度提高3倍，*9分鐘內(nèi)完成！ </p> <p> 產(chǎn)品特點(diǎn)： </p> <p> 1.速度快：9分鐘內(nèi)可獲得RNA。 </p> <p> 2.步驟少：4步即可完成。 </p> <p> 3.操作簡(jiǎn)單：SimplyP試劑盒內(nèi)只有四種試劑，*需經(jīng)過(guò)裂解、吸附過(guò)濾、洗滌、洗脫即可，提純整個(gè)操作過(guò)程簡(jiǎn)單易行，且提取的RNA質(zhì)量不受操作者熟練程度的影響。 </p> <p> 4.適用范圍廣：可用于組織、全血、體液、骨髓、細(xì)胞等樣品，且試劑盒中有可徹底滅活病原體的試劑，對(duì)于具有潛在傳染性的臨床樣本RNA的提取，有安全、可靠、不產(chǎn)生交叉污染的特殊優(yōu)勢(shì)。 </p> <p> 5.保質(zhì)期長(zhǎng)：由于本試劑盒采用的裂解液是中性的，沒有腐蝕性，且不易變質(zhì)，使得該試劑盒可室溫保存兩年。天津新手RNA提取試劑盒500T/5865元</p><p style="text-indent:25px">上海朝瑞生物科技有限公司是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司，致力于成為客戶業(yè)務(wù)創(chuàng)新、化工可信賴的合作伙伴。上海朝瑞科技擁有一支經(jīng)驗(yàn)豐富、技術(shù)創(chuàng)新的專業(yè)研發(fā)團(tuán)隊(duì)，以高度的專注和執(zhí)著為客戶提供[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]。公司擁有多年的行業(yè)經(jīng)驗(yàn)，每年以銷售收入達(dá)到2000-3000萬(wàn)元，如果您想了解更多產(chǎn)品信息，請(qǐng)通過(guò)頁(yè)面上的電話聯(lián)系我們。上海朝瑞科技始終關(guān)注自身，在風(fēng)云變化的時(shí)代，我們對(duì)自身的建設(shè)毫不懈怠，高度的專注與執(zhí)著使我們?cè)谛袠I(yè)的從容而自信。</p></p><br /><p>文章來(lái)源地址: 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