上海9分鐘完成RNA提取試劑盒50T/690元歡迎來電上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="按照每毫升**初的Trizol加入1ml75%乙醇（DEPC水配制）的比例，上海9分鐘完成RNA提取試劑盒50T/690元，Vortex混勻或顛倒混勻，讓沉淀懸浮起來以充分洗滌沉淀中的鹽分。9、4°C，12000rpm離心5分鐘，棄上清。剩余"><meta name="keywords" content="上海9分鐘完成RNA提取試劑盒50T/690元,RNA提取試劑盒"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" content="no-transform" /> <meta 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href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項目合作</a>?上海9分鐘完成RNA提取試劑盒50T/690元歡迎來電上海朝瑞生物科技供應(yīng)</p><div id="l289izb" class="leftbox clearfix"><h1>上海9分鐘完成RNA提取試劑盒50T/690元歡迎來電上海朝瑞生物科技供應(yīng)</h1><div id="xrjgdsi" class="proBig"><div id="83itz4i" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739419082031/9010268.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="5h3s7o3" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739419082031/9010268.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="sgdfrgs" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海9分鐘完成RNA提取試劑盒50T/690元,RNA提取試劑盒</p><p><span>***更新：</span>2020-06-30 13:19:31</p><p><span id="click" >瀏覽次數(shù)：1次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="rqdfhqx" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="jy2uoa3" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="zynevxo" class="box"><p><p> 按照每毫升**初的 Trizol 加入 1 ml 75%乙醇（DEPC 水配制）的比例，上海9分鐘完成RNA提取試劑盒50T/690元，Vortex混勻或顛倒混勻，讓沉淀懸浮起來以充分洗滌沉淀中的鹽分。 </p> <p> 9、4°C，12000rpm 離心 5 分鐘，棄上清。剩余的少量液體再用離心機(jī)瞬甩一下，徹底去掉上清液。注意不要吸到沉淀，否則會造成 RNA 損失。 </p> <p> 10、室溫放置空氣干燥 5-10 分鐘，上海9分鐘完成RNA提取試劑盒50T/690元，待 RNA 沉淀略干后，加入適量（約 20-100μl）的 RNase-free 水溶解 RNA 沉淀，-80°C 凍存?！咀ⅰ壳形鹱?RNA 徹底干燥，上海9分鐘完成RNA提取試劑盒50T/690元，否則將導(dǎo)致 RNA 溶解度降低。 </p> <p> <br /> 也導(dǎo)致基因檢測無法普及至基層醫(yī)療地區(qū)，甚至有的技術(shù)無法走出實(shí)驗(yàn)室應(yīng)用于臨床。上海9分鐘完成RNA提取試劑盒50T/690元</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海9分鐘完成RNA提取試劑盒50T/690元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739419082031/9010268.png"></div><p> 溶液 RL應(yīng)在2-8℃避光保存，其他溶液和純化柱室溫保存。 </p> <p> <br /> </p> <p> 注意事項 </p> <p> ● 嚴(yán)防操作環(huán)境、使用的容器、耗材和試劑的RNase污染。操作過程中勤換手套。 </p> <p> ● 使用本產(chǎn)品提取的RNA一般不含有DNA污染。在極少數(shù)情況下(與組織pH值等相關(guān))，如果有DNA污染而又必須去除，則可以用RNase-free的DNase處理樣品。 </p> <p> ● 請嚴(yán)格遵照操作步驟操作。 </p> <p> ● 不同起始材料試劑用量及預(yù)期RNA產(chǎn)率。 </p> ***次使用前應(yīng)在溶液 RPI、溶液 RW中加入無水乙醇，加入量請參見瓶子上的標(biāo)簽。 <p> <br /> 北京新手RNA提取試劑盒***的選擇所提取的RNA完整性好、純度高，可用于分子生物學(xué)后實(shí)驗(yàn)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海9分鐘完成RNA提取試劑盒50T/690元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739426933594/2190840.png"></div><p> 可選步驟： 4℃ 12,000 rpm (~13,400×g) 離心5分鐘，取上清，轉(zhuǎn)入一個新的無RNase 的離心管中。 </p> <p> 注意：如果樣品中含有較多蛋白、脂肪、多糖或肌肉、植物結(jié)節(jié)部分等，可加此步驟離心去除。離心得到的沉淀中包括細(xì)胞外膜、多糖、高分子量DNA，RNA存在于上清溶液中。 </p> <p> 加入200 μl 氯仿，蓋好管蓋，劇烈振蕩15 s，室溫放置3 min。 </p> <p> 4℃ 12,000 rpm (~13,400×g) 離心10 min，樣品會分成三層：黃色的有機(jī)相，中間層和無色的水相，RNA 主要在水相中，水相的體積約為所用溶液 RL試劑的60%。把水相轉(zhuǎn)移到新管中，進(jìn)行下一步操作。***次使用前應(yīng)在溶液 RPI、溶液 RW中加入無水乙醇，加入量請參見瓶上標(biāo)簽。 </p><p> 客戶提供： 1.需檢測的細(xì)胞或組織樣品，2.目的基因信息，3. 目的蛋白信息 4.WB一抗及抗體信息。**終交付：1.原始數(shù)據(jù)、結(jié)果圖片 2. 詳細(xì)的實(shí)驗(yàn)報告(含實(shí)驗(yàn)步驟、試劑耗材等) 3.蛋白質(zhì)譜報告。 </p> <p> RNA/DNA pull-down是檢測RNA/DNA結(jié)合蛋白與其靶RNA/DNA之間相互作用的主要實(shí)驗(yàn)手段之一。用生物素標(biāo)記RNA/DNA 探針，與胞漿蛋白提取液孵育，形成RNA/DNA—蛋白質(zhì)復(fù)合物。該復(fù)合物可與鏈親和素標(biāo)記的磁珠結(jié)合從而與孵育液中的其他成分分離。復(fù)合物洗脫后，通過Western Blot實(shí)驗(yàn)檢測特定的RNA/DNA結(jié)合蛋白是否與RNA/DNA相互作用，還可以對該蛋白進(jìn)行質(zhì)譜（MS）鑒定蛋白質(zhì)類型。當(dāng)前提取效果好的是RNA提取試劑盒，但其售價較高，單次實(shí)驗(yàn)費(fèi)用花費(fèi)太大。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海9分鐘完成RNA提取試劑盒50T/690元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739429267578/3679475.png"></div><p> 細(xì)胞中的RNA主要有三類：rRNA約占80％－85％，tRNA和核內(nèi)小分子RNA約占10%-15%，mRNA約占1%-5%，由于rRNA占絕大多數(shù)，因此我們用瓊脂糖凝膠電泳可以看到的即是rRNA，它也有三種類型：28S，18S，5S三條帶，在RNA的提取時，一定要注意RNase的污染，因RNA極易被RNase的降解，操作時應(yīng)盡量少說話，并在潔凈的環(huán)境中操作，主要防止手、唾液和空氣中Rnase的污染；另一方面使用的所有玻璃儀器、移液器、吸頭和離心管都用0.5%DEPC溶液處理過夜,然后高壓無菌15分鐘.但對于無菌的一次性使用的塑料制品基本上無RNase,，可以不經(jīng)處理直接用于提取RNA，實(shí)驗(yàn)室用的普通玻璃器皿和塑料制品經(jīng)常有Rnase污染，使用前必須180度干烤8小時或更長時間（玻璃器皿）或用氯仿沖洗（塑料制品），另外，由于材料不同，機(jī)體內(nèi)含有的糖、酚類物質(zhì)不一樣，因此材料不同用的方法也應(yīng)該不一樣。當(dāng)然，就RNA的提取而言，不一定試劑盒的提取效果就非常好。上海9分鐘完成RNA提取試劑盒50T/690元</p><p style="text-indent:25px">使用時無需配制其它試劑，非常方便，適合于RNA的快速提取。上海9分鐘完成RNA提取試劑盒50T/690元</p><p> RNA 降解可能的原因如下： </p> <p> 組織取出后沒有馬上進(jìn)行處理或冷凍，導(dǎo)致 RNase 的釋放降解樣品中的RNA。 </p> <p> 貼壁細(xì)胞在胰蛋白酶處理時被破壞。 </p> <p> 樣本量太大而加入的 Trizol 液少，導(dǎo)致裂解不完全，不能有效*** RNase的活性，進(jìn)而導(dǎo)致 RNA 降解。 </p> <p> 提取過程中使用的溶液、離心管或***頭未經(jīng) RNase 去除處理。 </p> <p> RNA 沉淀沒有保存于-80°C。 </p> <p> RNA 電泳時的電泳液及電泳槽中含有 RNase，或者電泳時電泳液溫度過高，造成 RNA 樣品被降解。 </p> <p> 樣品中蛋白和多糖含量高，建議加入氯仿之前先高速離心去除蛋白和多糖。上海9分鐘完成RNA提取試劑盒50T/690元</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)建于2003-01-22，注冊資金 700-1000萬元，辦公設(shè)施齊全，辦公環(huán)境優(yōu)越，已***實(shí)行網(wǎng)絡(luò)化辦公，**提高了速度和效率。致力于創(chuàng)造***的產(chǎn)品與服務(wù)，以誠信、敬業(yè)、進(jìn)取為宗旨，以建zrbiorise,SPRICELL,scienceladde明星產(chǎn)品為目標(biāo)，努力打造成為同行業(yè)中具有影響力的企業(yè)。公司不**提供專業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團(tuán)隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù),同時還建立了完善的售后服務(wù)體系,為客戶提供質(zhì)量的產(chǎn)品和服務(wù)。誠實(shí)、守信是對企業(yè)的經(jīng)營要求，也是我們做人的基本準(zhǔn)則。公司致力于打造***的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/447577.html</p></div></div></div></div></div><div id="98gi3pj" class="page-right twoColRt"><div id="cbykht2" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="393n2ov" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="8sgyugn" class="recomPic likePro"><a 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