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src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909832509766/1053763.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="w9subks" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909832509766/1053763.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="p5wnjcd" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>溫州數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR</p><p><span>***更新：</span>2020-07-01 20:19:42</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="3rsofoz" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="niwhdyu" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="zwofmdu" class="box"><p><p> 拷貝數(shù)分析基因拷貝數(shù)變異（Copy number variations，CNVs）是指人類(lèi)基因組中存在的，較之于參照基因組DNA片段缺失或復(fù)制大于1kb到Mb的亞微觀結(jié)構(gòu)變異，CNVs本身蘊(yùn)含著豐富的遺傳信息，對(duì)于研究基因變異在生物進(jìn)化及疾病進(jìn)程等領(lǐng)域具有重要意義。相較于現(xiàn)有的CNVs研究技術(shù)（定量PCR、高密度寡核苷酸芯片high </p> <p> density oligo nucleotide array，F(xiàn)ISH，比較基因組芯片microarray </p> <p> based comparative genomic hybridization，array-CGH，測(cè)序等），數(shù)字PCR在靈敏度和分辨率方面更具優(yōu)勢(shì)，尤其適用于更高拷貝數(shù)分析，確定確切的拷貝數(shù)，檢測(cè)精度超過(guò)±10%，溫州數(shù)字PCR定量檢測(cè)服務(wù)。它與計(jì)算器微電子技術(shù)、新材料，溫州數(shù)字PCR定量檢測(cè)服務(wù)，溫州數(shù)字PCR定量檢測(cè)服務(wù)、新能源、航天技術(shù)等被列為高科技，被認(rèn)為是21世紀(jì)科學(xué)技術(shù)的**。溫州數(shù)字PCR定量檢測(cè)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909832509766/1053763.jpg"></div><p style="text-indent:25px">微滴式數(shù)字PCR技術(shù),是將樣本通過(guò)微滴發(fā)生器進(jìn)行微滴化處理,制成約2104個(gè)1nL體積的微滴后進(jìn)行PCR反應(yīng)。PCR反應(yīng)結(jié)束后,使用微滴檢測(cè)器對(duì)每個(gè)微滴逐個(gè)檢測(cè),**終經(jīng)分析軟件計(jì)算出目標(biāo)DNA分子的濃度(copies/L)[2]。本研究選擇人mtDNA兩個(gè)編碼基因ND4和16SrRNA,設(shè)計(jì)特異性引物與探針,與檢測(cè)樣本制成反應(yīng)混合液,運(yùn)用微滴式數(shù)字PCR技術(shù),檢測(cè)陽(yáng)性反應(yīng)的微滴并計(jì)數(shù),達(dá)到同時(shí)進(jìn)行種屬鑒定和***定量的目的。1材料與方法1.1樣本制備1.1.1人mtDNAND4基因片段重組質(zhì)粒的制備和稀釋根據(jù)人mtDNAND4基因全長(zhǎng)參考序列天津石蠟包埋樣本數(shù)字PCRCt值就是指可以檢測(cè)到熒光值對(duì)應(yīng)的循環(huán)數(shù)；數(shù)字PCR是***的定量技術(shù)，基于單分子PCR方法來(lái)進(jìn)行計(jì)數(shù)的核酸。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909691865235/2119471.jpg"></div><p style="text-indent:25px">PCR擴(kuò)增過(guò)程在芯片上實(shí)現(xiàn)。**終對(duì)陽(yáng)性微滴計(jì)數(shù)從而得到精細(xì)的核酸***數(shù)量。作為***三個(gè)熒光通道檢測(cè)的數(shù)字PCR平臺(tái)，Naica? crystal數(shù)字PCR系統(tǒng)可在2小時(shí)內(nèi)實(shí)現(xiàn)多達(dá)36個(gè)目標(biāo)基因的檢測(cè)。Naica? Crystal系統(tǒng)由Geode微滴生成；擴(kuò)增儀和Prism3微滴閱讀分析系統(tǒng)組成，*需***耗材——Sapphire芯片，即可完成從加樣、微滴生成和擴(kuò)增，直到采集數(shù)據(jù)獲得結(jié)果。 r> Naica? crystal數(shù)字PCR可用于：基因表達(dá)差異檢測(cè)；拷貝數(shù)變異(CNV)；低豐度及稀有序列的精確定量；甲基化含量鑒定；*****的伴隨診斷；無(wú)創(chuàng)產(chǎn)前篩查；病原微生物的檢測(cè)(***、細(xì)菌等)；二代測(cè)序輔助建庫(kù)；*****的實(shí)時(shí)監(jiān)控 (ctDNA檢測(cè))等。</p><p> 科維思 </p> <p> 2017年5月27日，科維思DPCR分析系統(tǒng)進(jìn)入創(chuàng)新醫(yī)療器械特別審批程序，通過(guò)綠色通道，有望獲得快速審批。這款產(chǎn)品在進(jìn)口主流設(shè)備QuantStudio 3D數(shù)字PCR的基礎(chǔ)上，進(jìn)行了整個(gè)流程的系統(tǒng)化，實(shí)現(xiàn)了從科研到臨床的轉(zhuǎn)變。特別是針對(duì)正在接受靶向藥***的**患者，幫助臨床醫(yī)生及時(shí)發(fā)現(xiàn)患者體內(nèi)**細(xì)胞的抗藥性，調(diào)整診療方案，實(shí)現(xiàn)***效果的比較大化。 </p> <p> 2017年7月27日，國(guó)家食品藥品監(jiān)督管理總局（CFDA）正式批準(zhǔn)天津諾禾致源生物信息科技有限公司的數(shù)字PCR芯片閱讀儀——Digital PCR NG上市，同時(shí)明確該儀器適用于來(lái)源于人體的血漿游離DNA樣本中的T790M突變進(jìn)行檢測(cè)，后續(xù)可與諾禾致源正在進(jìn)行注冊(cè)申報(bào)的人EGFR基因T790M突變檢測(cè)試劑盒配合使用。定量PCR是依靠標(biāo)準(zhǔn)曲線或參照基因來(lái)測(cè)定核酸量，而數(shù)字PCR則讓你能夠直接數(shù)出DNA分子的個(gè)數(shù)，是對(duì)起始樣品的***定量。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909658583985/2801442.jpg"></div><p> 納流芯片的使用提供了便捷和直觀的機(jī)制來(lái)同時(shí)平行運(yùn)行上千個(gè)PCR反應(yīng)。每個(gè)孔都加入了樣品、擴(kuò)增混合物和 TaqMan測(cè)定試劑的混合物，然后進(jìn)行單獨(dú)分析以檢測(cè)存在（陽(yáng)性）或不存在（陰性）終點(diǎn)信號(hào)?？紤]到孔可能接收到多個(gè)靶標(biāo)序列分子，使用泊松模型應(yīng)用了一個(gè)校正因子。 </p> <p> 數(shù)字PCR的優(yōu)點(diǎn)無(wú)需依賴參考物或標(biāo)準(zhǔn)品通過(guò)使用更多PCR復(fù)制物能夠提高精確度對(duì)***劑具有較高耐受性能夠分析復(fù)雜混合物對(duì)較小倍數(shù)改變的線性檢測(cè) </p> <p> 數(shù)字PCR儀器和試劑能夠提高現(xiàn)有TaqMan測(cè)定的性能，從而實(shí)現(xiàn)更高的靈敏度、精度和***定量，使應(yīng)用從中獲益。一般大學(xué)都設(shè)在生命科學(xué)院內(nèi)，與生物技術(shù)，生物工程是兄弟專業(yè)。天津微滴式數(shù)字PCR</p><p style="text-indent:25px">其分支有生物分類(lèi)學(xué)、生理學(xué)、動(dòng)物學(xué)、植物學(xué)、微生物學(xué)、生物化學(xué)、遺傳學(xué)、分子生物學(xué)等。溫州數(shù)字PCR定量檢測(cè)服務(wù)</p><p> 數(shù)字PCR已經(jīng)被廣泛應(yīng)用到醫(yī)學(xué)、生物學(xué)等各個(gè)領(lǐng)域，如拷貝數(shù)變異、突變檢測(cè)、復(fù)雜來(lái)源樣品中低豐度核酸分子的檢測(cè)、NGS數(shù)據(jù)驗(yàn)證、miRNA等微小差異表達(dá)研究、單細(xì)胞基因表達(dá)分析等方面，在已知突變的**分子標(biāo)志物檢測(cè)、傳染病病原體檢測(cè)、基因組三倍體分析和基因表達(dá)分析等領(lǐng)域展現(xiàn)了強(qiáng)大的優(yōu)勢(shì)。 </p> <p> 德國(guó)Qioptiq iFLEX系列超穩(wěn)定低噪聲激光器模塊，是流式細(xì)胞儀、dPCR、共聚焦顯微等儀器理想的激光器模塊。此外，還可選擇光束組合器組合多波長(zhǎng)激光器，可以按照客戶定制要求提供圓光斑或整形光斑，并且功率可調(diào)。溫州數(shù)字PCR定量檢測(cè)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司注冊(cè)資金700-1000萬(wàn)元，是一家擁有5~10人***員工的企業(yè)。公司業(yè)務(wù)分為[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷(xiāo)售" ]等，目前不斷進(jìn)行創(chuàng)新和服務(wù)改進(jìn)，為客戶提供質(zhì)量的產(chǎn)品和服務(wù)。公司秉持誠(chéng)信為本的經(jīng)營(yíng)理念，在化工深耕多年，以技術(shù)為先導(dǎo)，以自主產(chǎn)品為**，發(fā)揮人才優(yōu)勢(shì)，打造化工質(zhì)量品牌。公司自成立以來(lái)發(fā)展迅速，業(yè)務(wù)不斷發(fā)展壯大，年?duì)I業(yè)額度達(dá)到2000-3000萬(wàn)元，未來(lái)我們將不斷進(jìn)行創(chuàng)新和改進(jìn)，讓企業(yè)發(fā)展再上新高。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/462221.html</p></div></div></div></div></div><div id="uav3t4o" class="page-right twoColRt"><div id="hvhqrie" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="jxtfbse" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="xqsoam3" class="recomPic likePro"><a 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