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08:10:20</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="s2mt3qs" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="h8bi8k3" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="nbyv0g9" class="box"><p><p> 9、堿性磷酸酶堿性磷酸酶（AKP），又稱堿性磷酸單酯酶，是酶組織化學(xué)中**常用的酶之一。分布***，常見于活躍運(yùn)輸?shù)哪ど?。該酶在堿性環(huán)境下能催化各種醇和酚的磷酸酯水解，**適pH為，可被Mg2+、Mn2+和某些氨基酸所***；**物、碘液、砷酸鹽等可***其活性。顯示AKP的方法很多，主要有鈣鈷法和同時(shí)偶聯(lián)法。前者所需試劑便宜，江蘇五色免疫組化，容易購(gòu)買，操作簡(jiǎn)單，但會(huì)出現(xiàn)假陽(yáng)性。后者方法較靈敏，而且在正常組織內(nèi)不存在萘酚，因此不會(huì)出現(xiàn)假陽(yáng)性，但所需試劑較難購(gòu)買。10、生物素（biotin）生物素是B族維生素之一。生物素在羧化，江蘇五色免疫組化、脫羧和轉(zhuǎn)羧化反應(yīng)中起輔酶作用。生物素是含硫水溶性維生素。廣布于動(dòng)物及植物組織，已從肝提取物和蛋黃中分離，是多種羧化酶輔基的成分。生物素標(biāo)記反應(yīng)簡(jiǎn)單、溫和且很少***抗體活性，生物素標(biāo)記的抗體可存放多年而不失活。能與生物素結(jié)合的二級(jí)試劑素，所需的各種標(biāo)記物均可購(gòu)置，江蘇五色免疫組化。此外，親和素和鏈霉親和素的結(jié)合背景水平非常低，結(jié)合緊密且快速，抗體很容易與經(jīng)過化學(xué)修飾的活化生物素結(jié)合。生物素又可以與結(jié)合有酶、熒光染料的親和素或鏈霉親和素連接，后者已有商品試劑供應(yīng)。這樣只需將純化抗體與生物素結(jié)合，就可用各種不同的標(biāo)記物來(lái)檢測(cè)。江蘇五色免疫組化</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇五色免疫組化,免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750849610/4353685.png"></div><p style="text-indent:25px">2. 免疫酶細(xì)胞化學(xué)技術(shù)是目前免疫組織化學(xué)研究中**常用的技術(shù)．基本原理是先以酶標(biāo)記的抗體與組織或細(xì)胞作用，然后加入酶的底物，生成有色的不溶性產(chǎn)物或具有一定電子密度的顆粒，通過光鏡或電鏡，對(duì)細(xì)胞或組織內(nèi)的相應(yīng)抗原進(jìn)行定位或定性研究．3. 免疫膠體金技術(shù)就是用膠體金標(biāo)記一抗，二抗或其他的能特異性的結(jié)合免疫球蛋白的分子（如葡萄球菌A蛋白）等作為探針對(duì)組織或細(xì)胞內(nèi)的抗原進(jìn)行定性，定位或定量研究．由于膠體金的電子密度高，多用于免疫電鏡的單標(biāo)記或多標(biāo)記的定位研究．江蘇五色免疫組化</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇五色免疫組化,免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899754550782/2606693.png"></div><p> –用戊二醛或碳化二亞胺作為交聯(lián)劑通過功能基團(tuán)-NH2、-COOH等將半抗原結(jié)合到載體上。結(jié)合比例為5kDa結(jié)合5～25個(gè)分子的小肽。免疫細(xì)胞化學(xué)技術(shù)抗體(antibody,Ab)1、抗體的概念：免疫球蛋白·機(jī)體受到抗原刺激后，由漿細(xì)胞合成并分泌出一類具有與抗原發(fā)生特異性結(jié)合的球蛋白，被稱為抗體。–抗體主要存在于血清內(nèi)；–抗體都是免疫球蛋白，但免疫球蛋白并不一定都是抗體。–免疫球蛋白根據(jù)重鏈的結(jié)構(gòu)及抗原特異性不同分為五種，既IgG、IgD、IgE、IgA、IgM。2、免疫組化實(shí)驗(yàn)中常用的抗體：?jiǎn)慰寺】贵w和多克隆抗體。·單克隆抗體：是一個(gè)B淋巴細(xì)胞克隆分泌的抗體，是應(yīng)用細(xì)胞融合雜交瘤技術(shù)免疫動(dòng)物制備的。–特異性強(qiáng)、抗體產(chǎn)量高?！ざ嗫寺】贵w：是將純化后的抗原直接免疫動(dòng)物后，從動(dòng)物血中所獲得的免疫血清，是多個(gè)B淋巴細(xì)胞克隆所產(chǎn)生的抗體混合物。–特異性低，會(huì)產(chǎn)生抗體的交叉反應(yīng)。–多克隆抗體廣泛應(yīng)用于石蠟包埋組織切片，可減少假陰性染色機(jī)會(huì)。–抗原與抗體的結(jié)合，要求量保持一定比例，當(dāng)抗原或抗體過量時(shí)，是不能聚合成大顆粒。3、抗體的制備——多克隆抗體的制備·動(dòng)物的選擇·佐劑(adjuvant)·免疫方法·免疫劑量·抗體效價(jià)的測(cè)定·放血或定期抽血。</p><p> 收藏查看我的收藏0有用+1已投票0免疫組織化學(xué)技術(shù)編輯鎖定免疫組織化學(xué)技術(shù)又稱為免疫細(xì)胞化學(xué)技術(shù)，是指用標(biāo)記的特異性抗原或抗體在組織細(xì)胞原位通過抗原抗體的免疫反應(yīng)和組織化學(xué)的呈色反應(yīng)，對(duì)相應(yīng)的抗原或抗體進(jìn)行定性、定位、定量測(cè)定的一項(xiàng)免疫學(xué)檢測(cè)方法。中文名免疫組織化學(xué)技術(shù)外文名Immunohistochemistrytechnique目錄1發(fā)展簡(jiǎn)史2現(xiàn)狀與展望免疫組織化學(xué)技術(shù)發(fā)展簡(jiǎn)史編輯免疫熒光組織化學(xué)是現(xiàn)***物學(xué)和醫(yī)學(xué)中廣泛應(yīng)用的技術(shù)之一，是由Coons和他的同事(1941)建立，免疫熒光技術(shù)與形態(tài)學(xué)技術(shù)相結(jié)合發(fā)展成免疫熒光細(xì)胞（或組織）化學(xué)。它與葡萄球菌A蛋白（SPA）、生物素與卵白素、植物血凝素（ConA等）相結(jié)合拓寬了領(lǐng)域；與激光技術(shù)、電子計(jì)算機(jī)，掃描電視和雙光子顯微鏡等技術(shù)結(jié)合發(fā)展為定量免疫熒光組織化學(xué)技術(shù)；熒光***細(xì)胞分類器Fluorescinactivatedcellsorter（FACS）的應(yīng)用，激光共聚焦顯微鏡的問世，使免疫熒光細(xì)胞技術(shù)發(fā)展到更高的階段，開創(chuàng)了免疫熒光技術(shù)的新領(lǐng)域。細(xì)胞顯微分光光度計(jì)與圖像分析儀的結(jié)合使免疫熒光組織化學(xué)的定量檢測(cè)更加準(zhǔn)確。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇五色免疫組化,免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899749443360/7546740.png"></div><p style="text-indent:25px"> 近年來(lái)，隨著免疫組織化學(xué)技術(shù)的發(fā)展和各種特異性抗體的出現(xiàn)，使許多疑難**得到了明確診斷。在常規(guī)**病理診斷中，5%-10%的病例單靠H.E.染色難以作出明確的形態(tài)學(xué)診斷。尤其是免疫組化在**診斷和鑒別診斷中的實(shí)用價(jià)值受到了普遍的認(rèn)可，其在低分化或未分化**的鑒別診斷時(shí)，準(zhǔn)確率可達(dá)50%-75%。免疫組織化學(xué)的臨床應(yīng)用主要包括以下幾方面：⑴惡性**的診斷與鑒別診斷；⑵確定轉(zhuǎn)移性惡性**的原發(fā)部位；⑶對(duì)某類**進(jìn)行進(jìn)一步的病理分型；⑷軟組織**的zhiliao一般需根據(jù)正確的組織學(xué)分類，因其種類多、組織形態(tài)相像，有時(shí)難以區(qū)分其組織來(lái)源，應(yīng)用多種標(biāo)志進(jìn)行免疫組化研究對(duì)軟組織**的診斷是不可缺少的；⑸發(fā)現(xiàn)微小轉(zhuǎn)移灶，有助于臨床zhiliao方案的確定，包括手術(shù)范圍的確定。⑹為臨床提供zhiliao方案的選擇。江蘇五色免疫組化</p><p style="text-indent:25px">江蘇五色免疫組化</p><p style="text-indent:25px">TSA技術(shù)(Tyramide Signal Amplification?，酪胺信號(hào)放大技術(shù))是做多重免疫熒光染色的一種多快好省的方法，它使同一張片子上所有的靶標(biāo)都可以選用特異性高的兔單克隆抗體，搭配同一支抗兔的HRP二抗就可以進(jìn)行實(shí)驗(yàn)，而且信號(hào)放大的倍數(shù)**增強(qiáng)。如果想要運(yùn)用這個(gè)技術(shù)，需要先做四個(gè)方面的準(zhǔn)備工作：嚴(yán)格驗(yàn)證的高特異性抗體：所有CST的IHC-P驗(yàn)證過的抗體都滿足mIHC(多重?zé)晒饷庖呓M化)高特異性的要求;HRP偶聯(lián)的針對(duì)一抗種屬亞型特異的二抗;酪胺熒光素(Perkin Elmer,Thermo Fisher Scientific等供應(yīng)商);多光譜成像系統(tǒng)(PerkinElmer等)。江蘇五色免疫組化</p><p style="text-indent:25px">上海朝瑞生物科技有限公司成立于2003-01-22，注冊(cè)資本：700-1000萬(wàn)元。該公司服務(wù)型的公司。公司是一家有限責(zé)任公司企業(yè)，以誠(chéng)信務(wù)實(shí)的創(chuàng)業(yè)精神、質(zhì)量高效的管理團(tuán)隊(duì)、精悍的職工隊(duì)伍，努力為廣大用戶提供***的產(chǎn)品。公司業(yè)務(wù)涵蓋[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]，價(jià)格合理，品質(zhì)有保證，深受廣大客戶的歡迎。上海朝瑞科技以創(chuàng)造***產(chǎn)品及服務(wù)的理念，打造高指標(biāo)的服務(wù)，引導(dǎo)行業(yè)的發(fā)展。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/760057.html</p></div></div></div></div></div><div id="t22r3i9" class="page-right twoColRt"><div id="oxuwgug" 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