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歡迎咨詢上海朝瑞生物科技供應(yīng)</h1><div id="i7hgita" class="proBig"><div id="lfmvryp" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909630185547/4431438.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="qpgfbx8" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909630185547/4431438.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="jnplxo7" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR</p><p><span>***更新：</span>2020-08-05 09:02:42</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="bkwtvho" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="fo7z3du" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="vk4kofh" class="box"><p><p> 整合“非靶向代謝組“***性”和靶向代謝組“準(zhǔn)確性”檢測技術(shù)優(yōu)點的新型代謝組檢測技術(shù)，具有高通量、超靈敏、廣覆蓋、定性定量準(zhǔn)等特點，千標(biāo)自建MWDB數(shù)據(jù)庫，可實現(xiàn)在生物樣本中一次性定性定量檢測600余種代謝物。 </p> <p> 樣本要求：1. 血清，江蘇專業(yè)數(shù)字PCR檢測服務(wù)、血漿：200ul/sample;液氮速凍-80℃保存，干冰寄送;應(yīng)盡量避免溶血。 2，江蘇專業(yè)數(shù)字PCR檢測服務(wù). 尿液：500 ul /sample;液氮速凍后-80℃保存，干冰寄送。 3. 組織：200mg/sample;液氮速凍后-80℃保存，干冰寄送。 4. 細(xì)胞：≥1×107/sample;液氮速凍后-80℃保存，干冰寄送。 5. 腦脊液：200 ul /sample;液氮速凍后-80℃保存，江蘇專業(yè)數(shù)字PCR檢測服務(wù)，干冰寄送。 6. 糞便及腸道內(nèi)容物：500mg/sample;液氮速凍后-80℃保存，干冰寄送。其他樣本類型請咨詢相關(guān)工作人員。需注意：避免反復(fù)凍融如今，你無需糾結(jié)了，因為數(shù)字PCR（digital PCR）來了。江蘇專業(yè)數(shù)字PCR檢測服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909630185547/4431438.jpg"></div><p> 數(shù)字PCR可對結(jié)核分枝桿菌耐藥突變檢測，從異質(zhì)性檢出能力、1%突變檢測穩(wěn)定性、平臺穩(wěn)定性、液滴數(shù)檢測、拷貝數(shù)檢測等多個技術(shù)指標(biāo)的對比。數(shù)字PCR應(yīng)用于1%的耐藥突變檢測具有優(yōu)勢。不同于傳統(tǒng)PCR，數(shù)字PCR能夠?qū)崿F(xiàn)定量，具有更加出色的靈敏度、特異性和精確性。其在基因表達(dá)、microRNA研究、基因組拷貝數(shù)鑒定、**標(biāo)志物稀有突變檢測、致病微生物鑒定、轉(zhuǎn)基因成分鑒定、NGS測序文庫精確定量和結(jié)果驗證等諸多方面具有的廣闊應(yīng)用前景。江蘇專業(yè)數(shù)字PCR檢測服務(wù)數(shù)字PCR技術(shù)不斷發(fā)展，Bio-Rad、LIFE Technologies及RainDance等廠家相繼推出技術(shù)較為成熟的數(shù)字PCR產(chǎn)品。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909728105469/8000575.jpg"></div><p style="text-indent:25px">微滴式數(shù)字PCR技術(shù),是將樣本通過微滴發(fā)生器進(jìn)行微滴化處理,制成約2104個1nL體積的微滴后進(jìn)行PCR反應(yīng)。PCR反應(yīng)結(jié)束后,使用微滴檢測器對每個微滴逐個檢測,**終經(jīng)分析軟件計算出目標(biāo)DNA分子的濃度(copies/L)[2]。本研究選擇人mtDNA兩個編碼基因ND4和16SrRNA,設(shè)計特異性引物與探針,與檢測樣本制成反應(yīng)混合液,運用微滴式數(shù)字PCR技術(shù),檢測陽性反應(yīng)的微滴并計數(shù),達(dá)到同時進(jìn)行種屬鑒定和***定量的目的。1材料與方法1.1樣本制備1.1.1人mtDNAND4基因片段重組質(zhì)粒的制備和稀釋根據(jù)人mtDNAND4基因全長參考序列</p><p> 數(shù)字PCR可對基因表達(dá)分析可對少量mRNA和miRNA的細(xì)微變化進(jìn)行準(zhǔn)確及重復(fù)性較好的檢測。環(huán)境監(jiān)測使用QX200系統(tǒng)可測試多種環(huán)境樣品，例如土壤和水。食品檢測采用經(jīng)過驗證的ddPCR方法對轉(zhuǎn)基因生物體(GMO)進(jìn)行評估。。Bio-Rad的技術(shù)主要來源于QuantaLife公司，QuantaLife利用油包水微滴生成技術(shù)開發(fā)了微滴式數(shù)字ddPCR，這是**早出現(xiàn)的相對成熟的數(shù)字PCR平臺，在運行成本和實驗結(jié)果穩(wěn)定性方面都基本達(dá)到了商品化的標(biāo)準(zhǔn)。2011年QuantaLife公司被Bio-Rad公司收購，其微滴式數(shù)字PCR儀產(chǎn)品更名為QX100，繼續(xù)在市場上銷售，2013年該公司又推出了升級型號QX200。具有較好的科學(xué)素養(yǎng)及一定的教學(xué)、科研能力。培養(yǎng)具備生物科學(xué)的基本理論。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110910158935547/2995932.jpg"></div><p> 數(shù)字PCR由于其定量的精確性和可靠性，在檢測領(lǐng)域具有廣闊的應(yīng)用空間。伴隨醫(yī)療技術(shù)發(fā)展，對于檢測要求不斷提高，數(shù)字PCR作為新一代檢測手段將迎來新發(fā)展和新增長。在數(shù)字PCR領(lǐng)域，國內(nèi)不少企業(yè)依托的是國外Quant Studio 3D技術(shù)，如果能夠突破國外技術(shù)壟斷，生產(chǎn)具有自主知識產(chǎn)權(quán)的儀器，將在技術(shù)源頭更具有優(yōu)勢。 </p> <p> 數(shù)字PCR是一種核酸分子***定量技術(shù)。是一個在模板DNA、引物(模板片段兩端的已知序列)和四種脫氧核苷酸等存在的情況下，DNA聚合酶依賴的酶促合成反應(yīng)，擴(kuò)增的特異性取決于引物與模板DNA的特異結(jié)合。相較于qPCR，BIOG數(shù)字PCR可讓你能夠直接數(shù)出DNA分子的個數(shù)，是對起始樣品的***定量。基本知識和較強(qiáng)的實驗技能，能在科研機(jī)構(gòu)、高等學(xué)校及企事業(yè)單位等從事科學(xué)研究。北京無創(chuàng)產(chǎn)前判斷數(shù)字PCR</p><p style="text-indent:25px">每個反應(yīng)器的核酸模板數(shù)少于或者等于1個。江蘇專業(yè)數(shù)字PCR檢測服務(wù)</p><p> 科維思 </p> <p> 2017年5月27日，科維思DPCR分析系統(tǒng)進(jìn)入創(chuàng)新醫(yī)療器械特別審批程序，通過綠色通道，有望獲得快速審批。這款產(chǎn)品在進(jìn)口主流設(shè)備QuantStudio 3D數(shù)字PCR的基礎(chǔ)上，進(jìn)行了整個流程的系統(tǒng)化，實現(xiàn)了從科研到臨床的轉(zhuǎn)變。特別是針對正在接受靶向藥***的**患者，幫助臨床醫(yī)生及時發(fā)現(xiàn)患者體內(nèi)**細(xì)胞的抗藥性，調(diào)整診療方案，實現(xiàn)***效果的比較大化。 </p> <p> 2017年7月27日，國家食品藥品監(jiān)督管理總局（CFDA）正式批準(zhǔn)天津諾禾致源生物信息科技有限公司的數(shù)字PCR芯片閱讀儀——Digital PCR NG上市，同時明確該儀器適用于來源于人體的血漿游離DNA樣本中的T790M突變進(jìn)行檢測，后續(xù)可與諾禾致源正在進(jìn)行注冊申報的人EGFR基因T790M突變檢測試劑盒配合使用。江蘇專業(yè)數(shù)字PCR檢測服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團(tuán)隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司，創(chuàng)建于2003-01-22。公司自2003-01-22成立以來，投身于[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]，是化工的主力軍。公司擁有多年的行業(yè)經(jīng)驗，每年以銷售收入達(dá)到2000-3000萬元，如果您想了解更多產(chǎn)品信息，請通過頁面上的電話聯(lián)系我們。上海朝瑞科技創(chuàng)始人仲緒軍，始終關(guān)注客戶，以優(yōu)化創(chuàng)新的科技，竭誠為客戶提供比較好的服務(wù)。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/814493.html</p></div></div></div></div></div><div id="8rykrdk" class="page-right twoColRt"><div id="lpj8t2e" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="mqugse2" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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