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href="http://www.cdcfah.com/zh/"><span>展會信息</span></a></li></ul></div></nav><section class="wrap clearfix"><section class="container clearfix tcPro"><section class="home-main container clearfix"><div id="dg43hiz" class="twoCol"><div id="8cjyzvm" class="page-proshow clearfix"><p class="current"> 當(dāng)前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項目合作</a>?北京多色熒光免疫組化分析服務(wù) 來電咨詢上海朝瑞生物科技供應(yīng)</p><div id="jbplhd7" class="leftbox clearfix"><h1>北京多色熒光免疫組化分析服務(wù) 來電咨詢上海朝瑞生物科技供應(yīng)</h1><div id="s37rbc4" class="proBig"><div id="k3923xj" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750849610/4353685.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="nmz24m3" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750849610/4353685.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="rjfryfo" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>北京多色熒光免疫組化分析服務(wù),免疫組化</p><p><span>***更新：</span>2020-08-09 19:05:56</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="2xip2py" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="btz8rnv" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="geamhyu" class="box"><p><p> ）問題及其解答：如何降低非特異性熒光染色和避免陰性著色？1、非特異性染色產(chǎn)生原因及其解決方案：⑴游離熒光素殘留在二抗中。一部分熒光素未與蛋白質(zhì)結(jié)合，形成了聚合物和衍化物，北京多色熒光免疫組化分析服務(wù)，而不能被透析除去。二抗配置時用透析法或?qū)游龇ǚ蛛x熒光素標(biāo)記的二抗和游離的二抗。購買高質(zhì)量、高純度的熒光素二抗。⑵抗體以外的血清蛋白與熒光素結(jié)合形成熒光素脲蛋白，可與組織成分結(jié)合。⑶組織抗原封閉不全。除去檢查的抗原以外，組織中還可能存在類屬抗原（如Forssman氏抗原），可與組織中特異性抗原以外之之相應(yīng)抗體結(jié)合。延長血清封閉時間。⑷從組織中難于提純抗原性物質(zhì)，所以制備的免疫血清中往往混雜一些抗其他組織成分的抗體，以致容易混淆。⑸抗體分子上標(biāo)記的熒光素分子太多，這種過量標(biāo)記的抗體分子帶過多的陰離子，北京多色熒光免疫組化分析服務(wù)，可吸附于正常組織上而呈現(xiàn)非特異性染色。⑹熒光素不純、標(biāo)本固定不當(dāng)?shù)取＂艘豢购投狗跤龡l件和濃度不合適。調(diào)整一抗和二抗孵育條件和濃度至比較好。⑻一抗和二抗孵育后的清洗不充分。增加清洗次數(shù)和延長清洗時間，北京多色熒光免疫組化分析服務(wù)。2、陰性染色產(chǎn)生的原因有：⑴一抗和二抗?jié)舛炔缓线m或孵育條件不妥。⑵熒光素提前衰退。北京多色熒光免疫組化分析服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京多色熒光免疫組化分析服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750849610/4353685.png"></div><p style="text-indent:25px"> 近年來，隨著免疫組織化學(xué)技術(shù)的發(fā)展和各種特異性抗體的出現(xiàn)，使許多疑難**得到了明確診斷。在常規(guī)**病理診斷中，5%-10%的病例單靠H.E.染色難以作出明確的形態(tài)學(xué)診斷。尤其是免疫組化在**診斷和鑒別診斷中的實用價值受到了普遍的認(rèn)可，其在低分化或未分化**的鑒別診斷時，準(zhǔn)確率可達(dá)50%-75%。免疫組織化學(xué)的臨床應(yīng)用主要包括以下幾方面：⑴惡性**的診斷與鑒別診斷；⑵確定轉(zhuǎn)移性惡性**的原發(fā)部位；⑶對某類**進(jìn)行進(jìn)一步的病理分型；⑷軟組織**的zhiliao一般需根據(jù)正確的組織學(xué)分類，因其種類多、組織形態(tài)相像，有時難以區(qū)分其組織來源，應(yīng)用多種標(biāo)志進(jìn)行免疫組化研究對軟組織**的診斷是不可缺少的；⑸發(fā)現(xiàn)微小轉(zhuǎn)移灶，有助于臨床zhiliao方案的確定，包括手術(shù)范圍的確定。⑹為臨床提供zhiliao方案的選擇。北京七色免疫組化</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京多色熒光免疫組化分析服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748701172/8129573.png"></div><p> –缺點：對封閉牢固的抗原決定簇暴露不理想。(3)微波照射法·將玻片放入裝有抗原修復(fù)液的容器中，置微波爐加熱至95℃以上，持續(xù)l0～15分鐘，冷卻后，按免疫組化染色步驟進(jìn)行?！ご朔椒ㄓ捎谖⒉▓鰞?nèi)極性分子、離子高速運(yùn)動，撞擊交聯(lián)的網(wǎng)鏈，使抗原異常的構(gòu)想恢復(fù)正常，且因分子運(yùn)動產(chǎn)熱、效率高、時間短，對抗原再現(xiàn)效果好。(4)高壓加熱法暴露抗原·將玻片浸入抗原修復(fù)液內(nèi)，置高壓鍋中高壓2～3min，可取得極好的效果。·由于高壓下受熱均勻，特別使用于大批量標(biāo)本的染色。(1)酸水解法·酸水解可使交聯(lián)斷裂、暴露抗原?！⒉Ｆ?mol/LHCl溶液中，室溫作用20min(溫度升高，作用時間縮短)?！ご朔茉鰪?qiáng)特異性染色，降低背景，但需注意水解過度將破壞抗原性及形態(tài)結(jié)構(gòu)。加熱法的注意事項·達(dá)到規(guī)定的溫度(92～95?C以上)；·維持一定的時間；·避免切片干涸(抗原可能完全丟失)；·加熱后必須經(jīng)過室溫自然冷卻20～30分鐘，使未折疊的蛋白分子鏈恢復(fù)天然構(gòu)型；·修復(fù)液：–**常用的是；–***研究表明堿性修復(fù)液更有效，推薦使用1mM的EDTA緩沖液()。4、載玻片的處理·抗原修復(fù)過程中，由于高溫、高壓等諸多固素的影響，極易造成脫片。為防預(yù)防脫發(fā)片，常用粘附劑處理載玻片。</p><p> pH)漂洗5min×3/次，不時震蕩(洗去多余游離的熒光素標(biāo)記的抗體)?！ぞ彌_甘油封片–分析純無熒光的甘油9份+pH，?！ょR檢：在熒光顯微鏡下觀察?！?yōu)點：方法簡便、特異性高，非特異性熒光染色少?！と秉c：敏感性偏低；而且每檢查一種抗原就需要制備一種熒光抗體。若檢測多種抗原需制備多種相應(yīng)的熒光標(biāo)記抗體。?直接免疫熒光法的注意事項·對熒光標(biāo)記的抗體的稀釋：要保證抗體的蛋白有一定的濃度；·一般稀釋度不應(yīng)超過1:20，抗體濃度過低，會導(dǎo)致產(chǎn)生的熒光過弱，影響結(jié)果的觀察。?直接免疫熒光法的注意事項(續(xù))·染色溫度和時間需要根據(jù)各種不同的標(biāo)本及抗原而變化；–染色時間：從10min到數(shù)小時，一般30min；–染色溫度：多采用室溫(25?C)，高于37?C可加強(qiáng)染色效果，但對不耐熱的抗原(如流行性乙型腦炎***)可采用0-2?C的低溫，延長染色時間。–低溫染色過夜較37?C30min效果好的多。?直接免疫熒光法的注意事項(續(xù))·試驗時需設(shè)置下列對照：–自發(fā)熒光對照(空白對照)：標(biāo)本加，。–陽性對照：用已知的陽性標(biāo)本加熒光標(biāo)記的特異性抗體。–特異性對照(***試驗)：標(biāo)本加未標(biāo)記的特異性抗體，再加熒光標(biāo)記的特異性抗體。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京多色熒光免疫組化分析服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751181641/619319.png"></div><p> 80年代到90年代相繼又有新的熒光素出現(xiàn)如R-藻紅朊、B-藻紅朊、C-藻青蛋白、cy2、cy3、cy5和cy7等均在流式細(xì)胞儀和激光共聚焦顯微鏡中廣泛應(yīng)用。由于免疫熒光組織化學(xué)的特異性，快速性和在細(xì)胞水平定位的準(zhǔn)確性，已在免疫學(xué)、微生物學(xué)、病理學(xué)、**學(xué)以及臨床檢驗等許多方面得到廣泛應(yīng)用，日益發(fā)揮重要作用。[1]免疫組織化學(xué)技術(shù)現(xiàn)狀與展望編輯免疫熒光組織化學(xué)技術(shù)經(jīng)過半個多世紀(jì)的不斷改進(jìn)和創(chuàng)新，已成為現(xiàn)代研究生物和醫(yī)學(xué)的重要手段之一。由于免疫熒光技術(shù)與形態(tài)、機(jī)能相結(jié)合不斷完善和發(fā)展，尤其是合成了多種新熒光素與抗體容易結(jié)合，且結(jié)合物穩(wěn)定。可以和FITC結(jié)合進(jìn)行免疫熒光組織化學(xué)雙標(biāo)記或三標(biāo)記。至今，它已和親合化學(xué)技術(shù)如SPA、Biotin以及avidin、ConA相結(jié)合，應(yīng)用領(lǐng)域也日益擴(kuò)大，又與現(xiàn)代的電子計算機(jī)、掃描電鏡技術(shù)、共聚焦顯微鏡、熒光***細(xì)胞分類器（FACS）以及數(shù)碼相機(jī)攝影技術(shù)的應(yīng)用，使得快速性、簡便性有了更大的提高，使得定量更加準(zhǔn)確。90年代又開展了熒光原位末端標(biāo)記和熒光原位雜交技術(shù)，使得應(yīng)用范圍更廣。雖然免疫組織化學(xué)技術(shù)有了很大的發(fā)展，如免疫金銀法、免疫膠體金法、SP、Evision二步法和CSA法，但由于它有自己獨特的優(yōu)點：特異性強(qiáng)。北京七色免疫組化</p><p style="text-indent:25px">北京多色熒光免疫組化分析服務(wù)</p><p> 這樣你才能大膽地去**和糾正一些錯誤的步驟，如抗體孵育條件主要是抗體濃度、溫度、時間，這三者一般是相互成反比的（相對），其中濃度是**重要的先決條件，溫度決定反應(yīng)的速度、時間決定反應(yīng)的量。比如溫度有4℃、室溫、37℃。我推薦4℃比較好，反應(yīng)**溫和，背景較淺；而37℃反應(yīng)速度較快，時間較短；室溫我不太提倡，除非你每次都把環(huán)境溫度控制在一定的范圍，否則，盡量選擇前兩者。2、定位和定性是免疫組化比較大的優(yōu)勢。相比于其他蛋白檢測方法，免疫組化具有定位較直接準(zhǔn)確、定性靈敏度高，是定位檢測分析優(yōu)先方法。尤其對于有些因子的轉(zhuǎn)位研究十分有用。3、免疫組化結(jié)果定量分析的前提是高質(zhì)量的染色切片。免疫組化結(jié)果也能定量分析，但必須是背景染色淺而特異性染色較深的情況下，分析**為準(zhǔn)確，這種原則可能也是我們?nèi)粘徃鍟r判定研究結(jié)果的必備條件。4、免疫組化實驗一定要設(shè)置陽性和陰性對照。陽性對照一般是用肯定表達(dá)這種抗原的切片來做；陰性對照一般是用PBS或非一抗替代一抗來進(jìn)行反應(yīng)，其余步驟均一致。前者是排除方法和實驗系統(tǒng)有無問題；后者是排除有無一抗外的非特異性染色。北京多色熒光免疫組化分析服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)建于2003-01-22，注冊資金 700-1000萬元，是一家專注1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團(tuán)隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司。公司目前擁有高技術(shù)人才5~10人人，以不斷增強(qiáng)企業(yè)核心競爭力，加快企業(yè)技術(shù)創(chuàng)新，實現(xiàn)穩(wěn)健生產(chǎn)經(jīng)營。公司以誠信為本，業(yè)務(wù)領(lǐng)域涵蓋[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]，我們本著對客戶負(fù)責(zé)，對員工負(fù)責(zé)，更是對公司發(fā)展負(fù)責(zé)的態(tài)度，爭取做到讓每位客戶滿意。公司憑著雄厚的技術(shù)力量、飽滿的工作態(tài)度、扎實的工作作風(fēng)、良好的職業(yè)道德，樹立了優(yōu)良的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]形象，贏得了社會各界的信任和認(rèn)可。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/862213.html</p></div></div></div></div></div><div id="dctp829" class="page-right twoColRt"><div id="8vmx7wc" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="y8t7zaw" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" 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