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歡迎咨詢上海朝瑞生物科技供應(yīng)</p><div id="uol7xro" class="leftbox clearfix"><h1>上海多色熒光免疫組化實驗服務(wù) 歡迎咨詢上海朝瑞生物科技供應(yīng)</h1><div id="xgncyv8" class="proBig"><div id="cgikmtv" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750458985/5221034.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="ec73asp" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750458985/5221034.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="ptlnub7" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海多色熒光免疫組化實驗服務(wù),免疫組化</p><p><span>***更新：</span>2020-08-16 23:08:33</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ra24phj" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="8eacj8y" class="page-proshow02"><p class="title">詳細說明</p><div id="ignkwdk" class="box"><p><p> 使許多疑難**得到了明確診斷。在常規(guī)**病理診斷中，5%-10%的病例單靠。尤其是免疫組化在**診斷和鑒別診斷中的實用價值受到了普遍的認可，其在低分化或未分化**的鑒別診斷時，準(zhǔn)確率可達50%-75%。免疫組織化學(xué)的臨床應(yīng)用主要包括以下幾方面：⑴惡性**的診斷與鑒別診斷；⑵確定轉(zhuǎn)移性惡性**的原發(fā)部位；⑶對某類**進行進一步的病理分型；⑷軟組織**的***一般需根據(jù)正確的組織學(xué)分類，因其種類多、組織形態(tài)相像，有時難以區(qū)分其組織來源，應(yīng)用多種標(biāo)志進行免疫組化研究對軟組織**的診斷是不可缺少的；⑸發(fā)現(xiàn)微小轉(zhuǎn)移灶，有助于臨床***方案的確定，包括手術(shù)范圍的確定。⑹為臨床提供***方案的選擇。免疫組化免疫組化鏡檢在鏡檢前可以通過相關(guān)資料查詢抗體的表達部位：細胞間質(zhì)、細胞膜、細胞漿、核膜、細胞核以及在其中的多個部位表達（如：MPO抗體表達在細胞膜、細胞漿，上海多色熒光免疫組化實驗服務(wù)、核膜、細胞核上）和表達組織（如：VWF抗體在血管壁上表達，呈現(xiàn)環(huán)形），上海多色熒光免疫組化實驗服務(wù)，上海多色熒光免疫組化實驗服務(wù)。在顯微鏡下觀察時，先在4倍鏡下查找組織及其范圍，然后查看整個組織確定陽性產(chǎn)物的表達部位，然后將陽性產(chǎn)物表達部位置于視野正**，換高倍鏡觀察。上海多色熒光免疫組化實驗服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海多色熒光免疫組化實驗服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750458985/5221034.png"></div><p> 問題及其解答：產(chǎn)生組織切片非特異性染色的原因有哪些？如何解決？1、抗體孵育時間過長、抗體濃度高易增加背景著色。這可通過縮短一抗/二抗孵育時間、稀釋抗體來控制。這是**重要的一條。2、一抗用多克隆抗體易出現(xiàn)非特異性著色，建議試用單克隆抗體看看。3、內(nèi)源性過氧化物酶和生物素在肝臟、腎臟等組織含量很高（含血細胞多的組織），需要通過延長滅活時間和增加滅活劑濃度來降低背景染色；4、非特異性組分與抗體結(jié)合，這需要通過延長二抗來源的動物免疫血清封閉時間和適當(dāng)增加濃度來加強封閉效果；5、DAB孵育時間過長或濃度過高；6、PBS沖洗不充分，殘留抗體結(jié)果增強著色，在一抗/二抗/SP孵育后的浸洗尤為重要；7、標(biāo)本染色過程中經(jīng)常出現(xiàn)干片，這容易增強非特異性著色。我的實際解決方案：以上的分析可能對于初學(xué)者還是不容易的，下面我就把我的排除實驗的具體過程與大家進行分享、交流和討論。1、首先排除抗體孵育條件。一般來說，著色效果的好壞與抗體的孵育條件是密不可分的，由于用SP法已經(jīng)做出來過一次且效果不錯，因此對于同樣組織的同一抗原進行分析，這種因素可以先排除。2、其次，DAB孵育時間是否太長？做出來那一次我是孵育8min，后來也是8-10min。天津六色免疫組化實驗服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海多色熒光免疫組化實驗服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751806641/2176473.png"></div><p> 時間過長，會使熒光減弱?！っ看卧囼灂r，需設(shè)置以下三種對照：–陽性對照：陽性血清+熒光標(biāo)記物–陰性對照：陰性血清+熒光標(biāo)記物–熒光標(biāo)記物對照：PBS+熒光標(biāo)記物?間接免疫熒光法的注意事項(續(xù))·標(biāo)本片需在操作的各個步驟中，始終保持濕潤，避免干燥?！ひ豢购投箲?yīng)始終保持在標(biāo)本片上，避免因放置不平使液體流失，從而造成非特異性熒光染色。免疫細胞化學(xué)技術(shù)免疫酶酶標(biāo)法【原理】·以酶作為標(biāo)記物與外加底物作用后產(chǎn)生不溶性色素，沉積于抗原和抗體反應(yīng)的部位；·酶降解底物的量與色澤濃度成正比?？煞从潮粶y定的抗原或抗體的量。1、常用的標(biāo)記酶及其顯色底物·辣根過氧化物酶(horseradishperoxidase,HRP)及底物·堿性磷酸酶(alkalinephosphatase,AP)及底物(1)辣根過氧化物酶(HRP)及底物·HRP是應(yīng)用**廣的一種酶，來源于植物辣根，由無色的酶蛋白和深棕色的鐵葉琳結(jié)合而成，分子量約40kDa，穩(wěn)定性好；·底物為過氧化物和供氫體(DH2)–過氧化物：常用過氧化氫和過氧化氫尿素。–供氫體：多用無色的還原型染料，通過反應(yīng)生成有色的氧化型染料，**常用的供氫體是DAB。DAB(二氨基聯(lián)苯胺)·DAB本身無色，反應(yīng)后呈棕色，不溶于水，不易褪色，電子密度高，**為常用。</p><p> –缺點：對封閉牢固的抗原決定簇暴露不理想。(3)微波照射法·將玻片放入裝有抗原修復(fù)液的容器中，置微波爐加熱至95℃以上，持續(xù)l0～15分鐘，冷卻后，按免疫組化染色步驟進行?！ご朔椒ㄓ捎谖⒉▓鰞?nèi)極性分子、離子高速運動，撞擊交聯(lián)的網(wǎng)鏈，使抗原異常的構(gòu)想恢復(fù)正常，且因分子運動產(chǎn)熱、效率高、時間短，對抗原再現(xiàn)效果好。(4)高壓加熱法暴露抗原·將玻片浸入抗原修復(fù)液內(nèi)，置高壓鍋中高壓2～3min，可取得極好的效果?！び捎诟邏合率軣峋鶆?，特別使用于大批量標(biāo)本的染色。(1)酸水解法·酸水解可使交聯(lián)斷裂、暴露抗原?！⒉Ｆ?mol/LHCl溶液中，室溫作用20min(溫度升高，作用時間縮短)。·此法能增強特異性染色，降低背景，但需注意水解過度將破壞抗原性及形態(tài)結(jié)構(gòu)。加熱法的注意事項·達到規(guī)定的溫度(92～95?C以上)；·維持一定的時間；·避免切片干涸(抗原可能完全丟失)；·加熱后必須經(jīng)過室溫自然冷卻20～30分鐘，使未折疊的蛋白分子鏈恢復(fù)天然構(gòu)型；·修復(fù)液：–**常用的是；–***研究表明堿性修復(fù)液更有效，推薦使用1mM的EDTA緩沖液()。4、載玻片的處理·抗原修復(fù)過程中，由于高溫、高壓等諸多固素的影響，極易造成脫片。為防預(yù)防脫發(fā)片，常用粘附劑處理載玻片。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海多色熒光免疫組化實驗服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748427735/7999973.png"></div><p> 是分泌性蛋白檢測優(yōu)先方法之一。下面介紹下免疫組化方法的具體實驗流程步驟。實驗流程簡介一、SP三步法1）石蠟切片，常規(guī)脫蠟至水。2)（無色液體）孵育10-30分鐘，以滅活內(nèi)源性過氧化物酶活性。3）蒸餾水沖洗，PBS浸泡5分鐘4）候選步驟：采用抗原修復(fù)：微波（建議30分鐘內(nèi)4次中火）、高壓、酶修復(fù)方法。自然冷卻，再用3分鐘×3次.5）血清封閉：室溫15-30分鐘，盡可能與二抗來源一致。傾去，勿洗。6）滴加適當(dāng)比例稀釋的一抗，37℃孵育2~3小時或4℃過夜（比較好復(fù)溫）。PBS沖洗，3分鐘×5次。7）滴加生物素標(biāo)記的二抗，室溫或37℃孵育30分鐘-1h。8)PBS沖洗，3分鐘×5次。9）滴加SP（鏈霉親和素-過氧化物酶），室溫或37℃孵育30分鐘-1h。10)PBS沖洗，3分鐘×5次。11）顯色劑顯色（DAB等）。12）自來水充分沖洗。13）可進行復(fù)染，脫水，透明。14）選擇適當(dāng)?shù)姆馄瑒┓馄?。二、即用型二步?）脫蠟、水化組織切片。2）根據(jù)所應(yīng)用的一抗的特殊要求，對組織切片進行預(yù)處理。3)，以阻斷內(nèi)源性過氧化物酶，PBS或TBS沖洗。4）滴加一抗，室溫或37℃孵育30~60分鐘，或4℃過夜，PBS或TBS浸洗3分鐘×5次。5）滴加enhangcer增強劑，37℃30min，PBS或TBS浸洗3分鐘×5次。上海多標(biāo)記免疫組化染色服務(wù)</p><p style="text-indent:25px">上海多色熒光免疫組化實驗服務(wù)</p><p> 免疫細胞化學(xué)技術(shù)組織印片·將潔凈載玻片輕壓于已暴露病灶的新鮮組織切面，細胞即粘附于玻片，晾干后浸入冷**或醋酸-乙醇固定10min，自然干燥后染片或-20℃保存。免疫細胞化學(xué)技術(shù)細胞培養(yǎng)片(細胞爬片)·貼壁細胞培養(yǎng)時，置蓋片于培養(yǎng)瓶中，使細胞在蓋片上生長，達適當(dāng)密度后取出固定(**-20?C固定10～20min)，再進行免疫染色。–蓋片的處理方法同載玻片的處理，但泡酸時間2h即可。–為了防止細胞脫片，可用多聚賴氨酸處理。免疫細胞化學(xué)技術(shù)細胞涂片·大多數(shù)細胞涂片由細胞懸液制成，包括：–血液、尿液、腦脊液；–體腔積液；–組織穿刺吸取，如骨髓、淋巴結(jié)或其他實質(zhì)性組織–懸浮培養(yǎng)的細胞或貼壁細胞經(jīng)消化后形成的懸液。免疫細胞化學(xué)技術(shù)細胞涂片(續(xù))·細胞涂片的方法：–手涂法·將細胞濃度調(diào)節(jié)到106/ml左右，可直接涂于載玻片上，但要均勻、不重疊。·圖片范圍應(yīng)小于1cm直徑，以節(jié)約試劑。–涂片機涂片法·將細胞樣品制成2×105～6/ml細胞懸液，吸取50～100?l(1～2×104～5cells)加入涂片機內(nèi)，1000rpm離心2min后細胞就均勻分布于玻片上。免疫細胞化學(xué)技術(shù)常用染色方法根據(jù)標(biāo)記物的不同分為免疫熒光法、免疫酶標(biāo)法、親和組織化學(xué)法。上海多色熒光免疫組化實驗服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司致力于化工，以科技創(chuàng)新實現(xiàn)***管理的追求。上海朝瑞科技作為1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的品牌企業(yè)，為客戶提供質(zhì)量的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]。公司擁有多年的行業(yè)經(jīng)驗，每年以銷售收入達到2000-3000萬元，如果您想了解更多產(chǎn)品信息，請通過頁面上的電話聯(lián)系我們。上海朝瑞科技創(chuàng)始人仲緒軍，始終關(guān)注客戶，以優(yōu)化創(chuàng)新的科技，竭誠為客戶提供比較好的服務(wù)。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/939107.html</p></div></div></div></div></div><div id="fyvnubd" class="page-right twoColRt"><div id="edfceln" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="cmtv3rb" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="eeg8ryq" 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