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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津數(shù)字PCR定量分析服務(wù),數(shù)字PCR</p><p><span>***更新：</span>2020-08-19 10:11:08</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="vtjrpvr" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話(huà): </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="hf7txvl" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="ndtrpxv" class="box"><p><p> 拷貝數(shù)分析基因拷貝數(shù)變異（Copy number variations，CNVs）是指人類(lèi)基因組中存在的，較之于參照基因組DNA片段缺失或復(fù)制大于1kb到Mb的亞微觀結(jié)構(gòu)變異，CNVs本身蘊(yùn)含著豐富的遺傳信息，對(duì)于研究基因變異在生物進(jìn)化及疾病進(jìn)程等領(lǐng)域具有重要意義。相較于現(xiàn)有的CNVs研究技術(shù)（定量PCR、高密度寡核苷酸芯片high </p> <p> density oligo nucleotide array，F(xiàn)ISH，比較基因組芯片microarray </p> <p> based comparative genomic hybridization，array-CGH，天津數(shù)字PCR定量分析服務(wù)，測(cè)序等），天津數(shù)字PCR定量分析服務(wù)，天津數(shù)字PCR定量分析服務(wù)，數(shù)字PCR在靈敏度和分辨率方面更具優(yōu)勢(shì)，尤其適用于更高拷貝數(shù)分析，確定確切的拷貝數(shù)，檢測(cè)精度超過(guò)±10%。了解相近專(zhuān)業(yè)的-般原理和知識(shí)。天津數(shù)字PCR定量分析服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津數(shù)字PCR定量分析服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909728105469/8000575.jpg"></div><p> 其實(shí)大家也不用太在意這么多可能連外星人都看不懂的檢測(cè)原理和對(duì)比分析，俗話(huà)說(shuō)得好，不看廣告看療效，是騾子是馬還得拉出來(lái)遛遛（偷笑）。 </p> <p> 數(shù)字PCR檢測(cè)其實(shí)離我們?cè)絹?lái)越近，下面我們以幾個(gè)臨床案例研究來(lái)舉例，展示數(shù)字PCR巨大的臨床應(yīng)用前景（敲黑板）。 </p> <p> **個(gè)體化診療 </p> <p> 通過(guò)檢測(cè)T790M位點(diǎn)突變情況，可以更好地評(píng)估一代TKI***的療效及耐藥情況并指導(dǎo)第三代TKI靶向藥Osimertinib的使用。然而，由于qPCR平臺(tái)ARMS檢測(cè)技術(shù)的靈敏度有限，沒(méi)有辦法在含大量EGFR基因野生型背景核酸中***準(zhǔn)確的檢測(cè)到T790M突變。這個(gè)時(shí)候dPCR技術(shù)展現(xiàn)出了****的檢測(cè)精度，此外，dPCR***定量的優(yōu)勢(shì)也能充分發(fā)揮出來(lái)了，可以監(jiān)控突變含量變化，做到及早發(fā)現(xiàn)耐藥。 </p> <p> <br /> 北京低靶點(diǎn)拷貝數(shù)字PCR生物科學(xué)是一門(mén)以實(shí)驗(yàn)為基礎(chǔ)，研究生命活動(dòng)規(guī)律的科學(xué)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津數(shù)字PCR定量分析服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909968974610/6529179.jpg"></div><p> **學(xué)研究是數(shù)字PCR的重要應(yīng)用領(lǐng)域，該技術(shù)作為極為重要的工具，能夠識(shí)別DNA突變（例如EGFR）、**患者用藥監(jiān)控、基因擴(kuò)增檢測(cè)、循環(huán)**細(xì)胞（CTC）特性研究、長(zhǎng)鏈非編碼RNA檢測(cè)、液體活檢中循環(huán)的核酸（cfDNA）和**細(xì)胞（CTC）的定量等研究中。 **標(biāo)志物稀有突變檢測(cè)在相比于野生型DNA，**相關(guān)的突變序列比例較低，經(jīng)常無(wú)法檢測(cè)。而憑借其超高靈敏度，dPCR系統(tǒng)可以很容易地定量分析低至0.001%-0.0001%的突變頻率。之前用任何方法都無(wú)法實(shí)現(xiàn)準(zhǔn)確穩(wěn)定檢測(cè)的樣本，現(xiàn)在可以使用dPCR輕松進(jìn)行定量分析。例如，已實(shí)現(xiàn)肺*相關(guān)的表皮生長(zhǎng)因子受體（EGFR）中T790M突變的檢測(cè)，可以更好地評(píng)估抗酪氨酸激酶抑劑的療效。 </p><p> 1985年美國(guó)科學(xué)家Kary Mullis發(fā)明PCR方法以后，PCR已經(jīng)成為生命科學(xué)研究領(lǐng)域中**常規(guī)的實(shí)驗(yàn)方法之一。PCR是用于放大擴(kuò)增特定的DNA片段的分子生物學(xué)技術(shù)，可看作是生物體外的特殊DNA復(fù)制。PCR的比較大特點(diǎn)，是能將微量DNA大量擴(kuò)增。 </p> <p> **傳統(tǒng)的一代PCR采用瓊脂糖電泳的方式對(duì)PCR產(chǎn)物進(jìn)行分析，操作繁瑣、難于做定量分析。為使PCR技術(shù)能對(duì)基因水平進(jìn)行定量分析，1992年開(kāi)發(fā)了熒光實(shí)時(shí)定量PCR（二代PCR）。 </p> <p> 在低拷貝靶分子、模板濃度差異的條件下，其檢測(cè)靈敏度、精確度都受到了限制。每個(gè)反應(yīng)器的核酸模板數(shù)少于或者等于1個(gè)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津數(shù)字PCR定量分析服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909832509766/1053763.jpg"></div><p style="text-indent:25px">QX200 Droplet數(shù)字PCR(Digital PCR) 系統(tǒng)的應(yīng)用領(lǐng)域： **生物標(biāo)志物研究和拷貝數(shù)變異分析以***的靈敏度和準(zhǔn)確度測(cè)量**突變的變異程度、檢測(cè)稀有的 DNA 靶拷貝以及解析拷貝數(shù)變異狀態(tài)。病原體檢測(cè) 精確地對(duì)靶 DNA 或 RNA 分子中的細(xì)微變化進(jìn)行定量分析，從而檢測(cè)和監(jiān)測(cè)病原體。與新一代測(cè)序無(wú)縫對(duì)接無(wú)需使用標(biāo)準(zhǔn)曲線，直接對(duì) NGS 庫(kù)執(zhí)行***定量分析。基因表達(dá)分析可對(duì)少量 mRNA 和 miRNA 的細(xì)微變化進(jìn)行準(zhǔn)確及重復(fù)性較好的檢測(cè)。環(huán)境監(jiān)測(cè) 使用 QX200 系統(tǒng)可測(cè)試多種環(huán)境樣品，例如土壤和水。食品檢測(cè) 采用經(jīng)過(guò)驗(yàn)證的 ddPCR 方法對(duì)轉(zhuǎn)基因生物體 (GMO) 進(jìn)行評(píng)估。他們的獨(dú)特優(yōu)勢(shì)是能夠產(chǎn)生非常均一、重復(fù)的1納升液滴。北京低靶點(diǎn)拷貝數(shù)字PCR</p><p style="text-indent:25px">數(shù)字PCR即Digital PCR（dPCR)，它是一種核酸分子***定量技術(shù)。天津數(shù)字PCR定量分析服務(wù)</p><p style="text-indent:25px">數(shù)字PCR是一種核酸分子***定量技術(shù)。當(dāng)前核酸分子的定量有三種方法，光度法基于核酸分子的吸光度來(lái)定量；實(shí)時(shí)熒光定量PCR（Real Time PCR）基于Ct值，Ct值就是指可以檢測(cè)到熒光值對(duì)應(yīng)的循環(huán)數(shù)；數(shù)字PCR是***的定量技術(shù)，基于單分子PCR方法來(lái)進(jìn)行計(jì)數(shù)的核酸定量，是一種***定量的方法。主要采用當(dāng)前分析化學(xué)熱門(mén)研究領(lǐng)域的微流控或微滴化方法，將大量稀釋后的核酸溶液分散至芯片的微反應(yīng)器或微滴中，每個(gè)反應(yīng)器的核酸模板數(shù)少于或者等于1個(gè)。天津數(shù)字PCR定量分析服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司位于上海市，注冊(cè)資本700-1000萬(wàn)元，旗下?lián)碛?~10人***專(zhuān)業(yè)的員工。zrbiorise,SPRICELL,scienceladde是上海朝瑞生物科技有限公司的主營(yíng)品牌，是專(zhuān)業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專(zhuān)業(yè)專(zhuān)長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車(chē)間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)公司，擁有自己**的技術(shù)體系。公司不**提供專(zhuān)業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專(zhuān)業(yè)專(zhuān)長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車(chē)間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù),同時(shí)還建立了完善的售后服務(wù)體系,為客戶(hù)提供質(zhì)量的產(chǎn)品和服務(wù)。自公司成立以來(lái)，一直秉承“以質(zhì)量求生存，以信譽(yù)求發(fā)展”的經(jīng)營(yíng)理念，始終堅(jiān)持以客戶(hù)的需求和滿(mǎn)意為**，為客戶(hù)提供質(zhì)量的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷(xiāo)售" ]，從而使公司不斷發(fā)展壯大。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/965791.html</p></div></div></div></div></div><div id="lrhflzf" class="page-right twoColRt"><div id="h7v7vbz" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="nvdbzx9" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" 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