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id="dqw3vw2" class="proBig"><div id="cpgxtpg" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751806641/2176473.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="nagho7k" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751806641/2176473.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="btzfwhi" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù),免疫組化</p><p><span>***更新：</span>2020-08-21 00:16:36</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ng8gmso" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="zm8k3zi" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="2tuqm2v" class="box"><p><p> 續(xù))·**過程中，動作要輕柔，盡量避免溶血·血液凝固后，及時離心收集血清，否則細(xì)胞溶解釋放的雜蛋白(例如蛋白水解酶)將污染抗體并將抗體水解，降低效價；·加疊氮化鈉，分裝，低溫保存，也可加一定的保護(hù)劑如BSA、甘油等。免疫細(xì)胞化學(xué)技術(shù)標(biāo)本的制備·標(biāo)本制備恰當(dāng)，是免疫組化成功的首要條件·免疫組化對組織和細(xì)胞標(biāo)本的要求–保持所檢標(biāo)本原有的結(jié)構(gòu)、形態(tài)；–在原位很大程度地保持待測抗原(或抗體)的免疫活性，既不淬滅、流失或彌散，也不被隱蔽，江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù)?！っ庖呓M化的組織和細(xì)胞標(biāo)本，制作流程與常規(guī)處理方法基本相同，但對組織，江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù)、細(xì)胞的處理又有其特殊要求及注意事項(xiàng)。–各種抗原由于其含量及特性的差異對標(biāo)本處理方式常有不同要求，因此要選擇適用于本實(shí)驗(yàn)的比較好方法。免疫組化中常用的組織和細(xì)胞標(biāo)本·組織標(biāo)本–石蠟切片–冰凍切片·細(xì)胞標(biāo)本–組織印片–細(xì)胞培養(yǎng)片(細(xì)胞爬片)–細(xì)胞涂片免疫細(xì)胞化學(xué)技術(shù)石蠟切片·石蠟切片是制作組織標(biāo)本**常用、**基本的方法–比較大優(yōu)點(diǎn)是組織形態(tài)保存好，且能作連續(xù)切片，有利于各種染色對照觀察；–石蠟塊還能長期存檔，江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù)，供回顧研究。·石蠟切片制作過程對組織內(nèi)抗原顯現(xiàn)有一定的影響，但可通過某些措施予以改善。江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751806641/2176473.png"></div><p> 免疫細(xì)胞化學(xué)技術(shù)抗原和抗體的要求·具有特異性高和親和力強(qiáng)的抗體是實(shí)驗(yàn)成功的首要條件。–對抗體的要求：純度高、比活性強(qiáng)；·高度特異性抗體的獲得，取決于抗原的純度。–對抗原的要求：純度高，免疫原性強(qiáng)，穩(wěn)定無變化。免疫細(xì)胞化學(xué)技術(shù)抗原(antigen,Ag)·抗原的概念：凡是在機(jī)體內(nèi)引起體液免疫和(或)細(xì)胞免疫反應(yīng)的物質(zhì)，稱為抗原?？乖哂袃蓚€方面的特性：–免疫原性：引起機(jī)體產(chǎn)生抗體和(或)致敏淋細(xì)胞的特性；–免疫反應(yīng)性：抗原能與相應(yīng)的抗體及致敏淋巴細(xì)胞發(fā)生特異的結(jié)合或反應(yīng)的特性?！じ鶕?jù)抗原是否顯示免疫原性分為：–完全抗原：分子量較大，一般在10kDa以上，并具有較復(fù)雜的化學(xué)組成?！っ庖咴?*強(qiáng)的是蛋白質(zhì)抗原，多糖次之；脂類和核酸必需和蛋白質(zhì)及多糖形成復(fù)合物才具有良好的免疫原性。–半抗原：又稱為不完全抗原，分子量較小。例如：某些短肽、多糖、類脂和***等?！ぐ肟乖匦枧c載體結(jié)合，才能獲得免疫原性。載體·通常是具有高度免疫原性的大分子物質(zhì)，具有將免疫原性傳遞給耦聯(lián)的半抗原能力。–常用的載體有鑰孔血藍(lán)蛋白(keyholelimpethemocyanin，KLH)、牛血清白蛋白(bovineserumalbumin,BSA)、卵白蛋白(Ovalbumin，OVA)等。北京五色免疫組化分析服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750380860/9179133.png"></div><p> <p> 為了在同一張組織切片或細(xì)胞學(xué)涂片上同時或先后顯示兩種或兩種以上的抗原，發(fā)展了雙重和多重免疫組化技術(shù)。這對于在原位了解不同抗原的關(guān)系，將形態(tài)與功能相結(jié)合極為有用。 </p> <p> 近年來，雙重標(biāo)記技術(shù)結(jié)合了免疫組化和核酸分子雜交技術(shù)，已跨越蛋白質(zhì)水平，進(jìn)入DNA、RNA水平，可在同一細(xì)胞內(nèi)顯示兩種不同的DNA或mRNA，或者顯示DNA和蛋白質(zhì)。也有人以放射性同位素標(biāo)記和酶組織化學(xué)結(jié)合來顯示靶DNA和抗原。雙重染色的基本方法有雙重?zé)晒夂碗p重酶標(biāo)記法。雙重?zé)晒鈽?biāo)記**常用的是FITC和TMRITC或RB200，雙重酶標(biāo)記常用的是辣根過氧化物酶和堿性磷酸酶。 </p> </p><p> 在室溫下孵育5分鐘以封閉非特異性的背景染色。（注：孵育不要超過10分鐘，否則會導(dǎo)致特異性染色降低。如果一抗的稀釋液中含有5－10%正常羊血清，這一步可以省略。）6.緩沖液洗5min/2次。7.滴加一抗工作液37℃孵育1－2小時。（具體孵育時間和溫度由試驗(yàn)者**終決定）8.緩沖液洗5min/2次。9．滴加PrimaryAntibodyEnhancer（增強(qiáng)子），在室溫下孵育20分鐘。10．緩沖液洗5min/2次。11．滴加HRPPolymer（酶標(biāo)二抗），在室溫下孵育30分鐘。（注：HRPPolymer對光敏感，應(yīng)避免不必要的光暴露并儲存在不透明的小瓶中。）12．緩沖液洗5min/2次。13．向1mlDABPlusSubstrate（或AECPlusSubstrate)中滴加1-2滴DABPlusChromogen（或AECPlusChromogen），混勻后滴加到切片上，孵育3－15分鐘。（具體時間由染色深淺決定。）14．自來水充分沖洗，復(fù)染，脫水，透明，封片。免疫組化操作步驟⑴、石蠟切片脫蠟至水。⑵、3%H2O2室溫孵育5-10分鐘，以消除內(nèi)源性過氧化物酶的活性。⑶、蒸餾水沖洗，PBS浸泡5分鐘x2（如需抗原修復(fù)，可在此步后進(jìn)行）。⑷、5-10%正常山羊血清（PBS稀釋）封閉，室溫孵育10分鐘，傾去血清，勿洗。滴加一抗工作液，37℃孵育1-2小時或4℃過夜。⑸、PBS沖洗，5分鐘x3次。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899747812500/5201169.png"></div><p> 用甲醇配置過氧化氫比雙蒸水或PBS可能好在保護(hù)抗原和固定組織作用，過氧化氫孵育時間過長易引起脫片；現(xiàn)用現(xiàn)配，配好后4℃避光保存。不過，現(xiàn)在已有“第二代即用型免疫組化試劑盒”避免內(nèi)源性生物素的干擾，推薦使用。7）血清封閉組織切片上有剩余的位點(diǎn)可以與一抗非特異性結(jié)合，造成后續(xù)結(jié)果的假陽性；封閉血清一般是和二抗同一來源的，血清中動物自身的抗體，預(yù)先能和組織中有交叉反應(yīng)的位點(diǎn)發(fā)生結(jié)合；也可以用小牛血清、BSA、羊血清等，但不能與一抗來源一致。一般室溫10-30min。但也要防止封閉過度8）一抗和二抗?jié)舛群头跤龝r間一抗孵育條件在免疫組化反應(yīng)中**重要，包括孵育時間、溫度和抗體濃度。一抗孵育溫度有幾種：4℃、室溫、37℃，其中4℃效果比較好；孵育時間：這與溫度、抗體濃度有關(guān)，一般37℃1-2h，而4℃過夜和從冰箱拿出后37℃復(fù)溫45min。具體條件還要摸索。二抗孵育條件：二抗一般室溫或37℃30min-1h，具體時間需要摸索，而濃度一般有工作液，若是濃縮液還要摸索濃度。但在免疫組化中我們一般先把二抗?jié)舛群头跤龝r間先定下，然后去摸索一抗?jié)舛群头跤龝r間。建議一抗反應(yīng)在4℃比較好，反應(yīng)溫和，但時間比較好超過16~24h。上海四色免疫組化技術(shù)服務(wù)</p><p style="text-indent:25px">江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù)</p><p> ⑴抗原有無丟失主要看組織切片的新鮮程度，一般切片室溫保存超過3-6個月，可能切片內(nèi)的抗原丟失很嚴(yán)重（有文獻(xiàn)支持），此時可以通過重新用石蠟塊切片來進(jìn)一步驗(yàn)證（蠟塊需要低溫保存）。⑵石蠟切片在制作過程中可能因醛基對抗原決定族的封閉，這需要通過抗原修復(fù)來充分暴露，從而增加抗原抗體結(jié)合反應(yīng)，提高陽性率，我一般用6min*4次中火微波抗原修復(fù)，用枸櫞酸鈉緩沖液。⑶組織切片中本身抗原含量的多少？這方面我有教訓(xùn)的，我做胎鼠睪丸間質(zhì)細(xì)胞鑒定，用1：200一抗效果很好；但我用1：200一抗孵育成年睪丸間質(zhì)細(xì)胞檢測，幾乎呈陰性，后來證實(shí)是因?yàn)閮烧呖乖肯嗖钌踹h(yuǎn)，則一抗也要適當(dāng)提高濃度。2、其次，檢查抗體有無選擇錯誤和抗體孵育條件是否不適。⑴一抗選擇單克隆抗體易出現(xiàn)陰性結(jié)果，因?yàn)殪`敏度低但特異性好；一抗的speciesreactivity中無檢測組織的種屬，這是比較常見的錯誤；一抗選擇rat，而二抗是抗mouse/rabbit等均有可能出現(xiàn)陰性結(jié)果。⑵抗體孵育時間過短，容易導(dǎo)致陰性結(jié)果。一般一抗我建議4℃孵育過夜和37℃復(fù)溫45min；二抗我一般37℃30min。我不喜歡參照二抗染色試劑盒說明書。⑶抗體濃度過低。這是陰性結(jié)果的**可能原因。必須提高稀釋度。江蘇多色熒光免疫組化實(shí)驗(yàn)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司坐落在上海市松江區(qū)廣富林路697號昂立大廈2113室，是一家專業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)公司。公司目前擁有5~10人員工，為員工提供廣闊的發(fā)展平臺與成長空間，為客戶提供高質(zhì)高速的產(chǎn)品服務(wù)，深受員工與客戶好評。上海朝瑞生物科技有限公司主營業(yè)務(wù)涵蓋[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]，堅(jiān)持“質(zhì)量優(yōu)先、質(zhì)量服務(wù)、顧客滿意”的質(zhì)量方針，贏得廣大客戶的支持和信賴。一直以來公司堅(jiān)持以客戶為中心、[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]市場為導(dǎo)向，重信譽(yù)，保質(zhì)量，想客戶之所想，急用戶之所急，全力以赴滿足客戶的一切需要。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/984252.html</p></div></div></div></div></div><div id="83mcdpq" class="page-right twoColRt"><div id="cwxokgh" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="3jkbxyj" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 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