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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津數(shù)字PCR技術(shù)服務(wù),數(shù)字PCR</p><p><span>***更新：</span>2020-08-22 09:06:23</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="t7jxgzd" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="17rb7qj" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="c67u3og" class="box"><p><p> 數(shù)字PCR由于檢測(cè)時(shí)完全不依賴傳統(tǒng)的Ct值即可實(shí)現(xiàn)真正意義上的精確定量，因而可以提供比實(shí)時(shí)熒光定量PCR更精確的基因差異表達(dá)研究，尤其對(duì)于那些靶基因表達(dá)差異微小的情況，如：mRNA、microRNA、lncRNAs等的表達(dá)分析；等位基因的不平衡表達(dá)；單細(xì)胞基因表達(dá)分析；外泌體核酸分子定量分析等。數(shù)字PCR微滴化的樣品處理及檢測(cè)，這種擺脫Ct值的計(jì)算方法而直接獲取目標(biāo)基因的精確拷貝數(shù)，為拷貝數(shù)變異的研究提供了精確的檢測(cè)精度，天津數(shù)字PCR技術(shù)服務(wù)，天津數(shù)字PCR技術(shù)服務(wù)。是其他諸如二代測(cè)序、芯片雜交等平臺(tái)無(wú)法達(dá)到的。采用數(shù)字PCR能夠有效對(duì)二代測(cè)序（NGS）和微陣列比較基因組雜交（aCGH）等實(shí)驗(yàn)結(jié)果進(jìn)行驗(yàn)證，并具有檢測(cè)周期短、成本低、樣本通量高等特點(diǎn)可用于拷貝數(shù)變異（CNV）研究，天津數(shù)字PCR技術(shù)服務(wù)。生物科學(xué)是一門前沿的邊緣學(xué)科，要想在此有成就，深造是難免的。天津數(shù)字PCR技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津數(shù)字PCR技術(shù)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909586328125/5976755.jpg"></div><p> 泛生子 </p> <p> 2017年10月31日，國(guó)家食品藥品監(jiān)督管理總局（CFDA）正式批準(zhǔn)重慶泛生子生物科技有限公司的生物芯片閱讀儀（Digital PCR）——GENETRON 3D上市。在已上市取得CFDA批準(zhǔn)的數(shù)字PCR儀器中，泛生子GENETRON 3D適用范圍更加***，且無(wú)配套試劑盒限定。 </p> <p> 在全球數(shù)字PCR領(lǐng)域，無(wú)論是技術(shù)水平還是公司規(guī)模，Bio-Rad和Thermo Fisher兩家大型企業(yè) </p> <p> 泛生子生物芯片閱讀儀（Digital PCR）是泛生子與科學(xué)服務(wù)領(lǐng)域的世界***——Thermo Fisher （賽默飛世爾）共同合作開發(fā)，該系統(tǒng)速度快、檢測(cè)精細(xì)、性能穩(wěn)定、操作簡(jiǎn)單，可***應(yīng)用于醫(yī)療機(jī)構(gòu)、臨檢中心和科研院所等，更好地服務(wù)于中國(guó)臨床檢測(cè)及科研需求。天津數(shù)字PCR技術(shù)服務(wù)近***物技術(shù)的技術(shù)特征是微生物發(fā)酵技術(shù)，現(xiàn)***物技術(shù)的技術(shù)特征就是以基因工程為首要標(biāo)志。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津數(shù)字PCR技術(shù)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909968974610/6529179.jpg"></div><p style="text-indent:25px">Bio-Rad的技術(shù)主要來(lái)源于QuantaLife公司，QuantaLife 利用油包水微滴生成技術(shù)開發(fā)了微滴式數(shù)字PCR技術(shù)，這也是**早出現(xiàn)的相對(duì)成熟的數(shù)字PCR平臺(tái)，在運(yùn)行成本和實(shí)驗(yàn)結(jié)果穩(wěn)定性方面都基本達(dá)到了商品化的標(biāo)準(zhǔn)。2011年，QuantaLife 公司被Bio-Rad公司收購(gòu)，其微滴式數(shù)字PCR儀產(chǎn)品更名為QX100型號(hào)儀繼續(xù)在市場(chǎng)上銷售，這個(gè)早期型號(hào)為dPCR概念的普及和應(yīng)用領(lǐng)域的拓展發(fā)揮了重要作用。2013年該公司又推出了升級(jí)型號(hào)QX200。1.Bio-Rad國(guó)外企業(yè)</p><p> 基因表達(dá)分析可對(duì)少量 mRNA 和 miRNA 的細(xì)微變化進(jìn)行準(zhǔn)確及重復(fù)性較好的檢測(cè)。環(huán)境監(jiān)測(cè)使用 QX200 系統(tǒng)可測(cè)試多種環(huán)境樣品，例如土壤和水。食品檢測(cè)采用經(jīng)過(guò)驗(yàn)證的 ddPCR 方法對(duì)轉(zhuǎn)基因生物體 (GMO) 進(jìn)行評(píng)估。 </p> <p> 將初始的包含有目標(biāo)基因、引物、探針及聚合酶的PCR樣品進(jìn)行稀釋后，等分到幾十到幾十萬(wàn)分的**反應(yīng)單元中，在每個(gè)反應(yīng)單元中包含0或1個(gè)靶DNA分子模板，對(duì)所有反應(yīng)單元進(jìn)行PCR擴(kuò)增反應(yīng)。反應(yīng)結(jié)束后，對(duì)每個(gè)單元進(jìn)行熒光檢測(cè)，并將具有熒光信號(hào)的單元標(biāo)記為“1”（陽(yáng)性），無(wú)熒光信號(hào)的標(biāo)記為“0”（陰性），直接統(tǒng)計(jì)陰、陽(yáng)性反應(yīng)單元數(shù)量，即可計(jì)算出原始溶液中DNA模板拷貝數(shù)。 DNA聚合酶依賴的酶促合成反應(yīng)，擴(kuò)增的特異性取決于引物與模板DNA的特異結(jié)合。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津數(shù)字PCR技術(shù)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909800390625/81991.jpg"></div><p> <br /> </p> <p> RainDance于2012年推出Raindrop型號(hào)數(shù)字PCR，能夠提供更高的檢測(cè)動(dòng)態(tài)范圍，適用于處理更大濃度差異的不同樣品，這個(gè)設(shè)備將其原有的二代測(cè)序文庫(kù)制備平臺(tái)技術(shù)平移到數(shù)字PCR技術(shù)平臺(tái)，在高壓氣體驅(qū)動(dòng)下，將每個(gè)標(biāo)準(zhǔn)反應(yīng)體系分割成包含100萬(wàn)至1000萬(wàn)個(gè)皮升級(jí)別微滴的反應(yīng)乳液，該公司的創(chuàng)始人之一Darren Link表示這種超高的微滴數(shù)目可以為用戶“提供更高的檢測(cè)動(dòng)態(tài)范圍，適用于處理更大濃度差異的不同樣品”。在2017年Bio-Rad完成了對(duì)RainDance公司的收購(gòu)，可以視作數(shù)字PCR領(lǐng)域的強(qiáng)強(qiáng)聯(lián)合。他們的獨(dú)特優(yōu)勢(shì)是能夠產(chǎn)生非常均一、重復(fù)的1納升液滴。天津數(shù)字PCR技術(shù)服務(wù)</p><p style="text-indent:25px">在定量PCR時(shí)，我們常常糾結(jié)一個(gè)問(wèn)題，究竟是相對(duì)定量還是***定量呢？天津數(shù)字PCR技術(shù)服務(wù)</p><p> 數(shù)字PCR實(shí)際上是一個(gè)在模板DNA、引物(模板片段兩端的已知序列)和四種脫氧核苷酸等存在的情況下，DNA聚合酶依賴的酶促合成反應(yīng)，擴(kuò)增的特異性取決于引物與模板DNA的特異結(jié)合。bio-radQX200微滴式數(shù)字PCR(ddPCR)系統(tǒng)可對(duì)DNA或RNA分子進(jìn)行定量分析，QX200Droplet數(shù)字PCR(DigitalPCR)系統(tǒng)的應(yīng)用領(lǐng)域：**生物標(biāo)志物研究和拷貝數(shù)變異分析以高靈敏度和準(zhǔn)確度測(cè)量**突變的變異程度、檢測(cè)稀有的DNA靶拷貝以及解析拷貝數(shù)變異狀態(tài)。病原體檢測(cè)精確地對(duì)靶DNA或RNA分子中的細(xì)微變化進(jìn)行定量分析，從而檢測(cè)和監(jiān)測(cè)病原體。與新一代測(cè)序無(wú)縫對(duì)接無(wú)需使用標(biāo)準(zhǔn)曲線，直接對(duì)NGS庫(kù)執(zhí)行定量分析。天津數(shù)字PCR技術(shù)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)建于2003-01-22，注冊(cè)資金 700-1000萬(wàn)元，辦公設(shè)施齊全，辦公環(huán)境優(yōu)越，已全力實(shí)行網(wǎng)絡(luò)化辦公，**提高了速度和效率。zrbiorise,SPRICELL,scienceladde是上海朝瑞生物科技有限公司的主營(yíng)品牌，是專業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 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