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src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336147089843/7803549.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="32ewys2" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產品關鍵詞：</span>D-泛解酸內酯推薦產品</p><p><span>***更新：</span>2020-06-19 19:28:12</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="8qp8g2r" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網址: </span></p><p><span>手機: </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="yszbykz" class="page-proshow02"><p class="title">詳細說明</p><div id="gkmoq82" class="box"><p><p style="text-indent:25px">KATAOKA M 等于1992 年分離純化出星狀諾卡氏菌生產的L-泛解酸內酯脫氫酶，但比酶活只有0.041 7 U/mg。該菌的基因組信息已被披露，然而至今未有構建其L-泛解酸內酯脫氫酶基因工程菌的報道。2009 年，王永澤等利用面包酵母生產酮泛解酸內酯還原酶，并利用β-環(huán)糊精結合底物，減少底物對菌體的0作用，終產物得率達45%，光學純度達到90%以上。SI D等于2012 年在嗜麥芽寡養(yǎng)單孢菌845 中克隆一個大小約為30 kDa 的酮泛解酸還原酶，D-泛解酸內酯推薦產品，并與葡萄糖脫氫酶共表達成大腸工程菌，D-泛解酸內酯推薦產品，D-泛解酸內酯推薦產品，構建輔酶循環(huán)體系。該大腸工程菌的酮泛解酸還原酶的比酶活達到27.7 U/mg。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="D-泛解酸內酯推薦產品" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336147089843/7803549.png"></div><p style="text-indent:25px">“微生物酶法拆分制備D－泛解酸內酯及用于生產D-泛酸鈣與D-泛醇”項目選育了一株能高產立體專一性D-泛解酸內酯水解酶、不利用不降解泛解酸內酯或泛解酸的微生物菌株串珠鐮孢霉，建立了國際**的霉菌交聯(lián)原位固定化方法，酶轉化時間短到3～5小時，效率高，反復分批酶轉化可達180次以上，所得到的酶水解產物光學純度達到99％e．e．以上，因此具有較好的技術經濟競爭力。該項目采用的D-泛酸鈣生產能力微生物酶催化拆分方法，與傳統(tǒng)化學拆分方法相比，以生產D-泛醇為例，原材料消耗減少69．2％，廢液、廢渣排放分別減少65．5％、43．8％，能耗減少12．7％，生產成本降低了26．5％，同時，生物法改善了操作環(huán)境，提高了產品品質與產品安全性，提高了D-泛酸生產的整體技術水平，達到了用高新生物技術改造傳統(tǒng)化學工業(yè)的目的。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="D-泛解酸內酯推薦產品" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336263828125/6823745.png"></div><p> D-泛解酸內酯水解酶基因,基因片段長度為1146bp,該序列同來源于尖鐮孢霉菌(F.oxysporum)AKU 3702菌株的編碼D-泛解酸內酯水解酶cDNA結構基因的同源性為90.06%.將所得片段定向克隆到pTrc99a載體中,轉化至JM109感受態(tài)細胞,篩選出了陽性克隆.經IPTG誘導陽性菌,進行SDS-PAGE電泳,檢測出在約40kD處有一蛋白表達帶.對兩株重組基因工程菌的比活力進行測定,結果分別為37U和41U. </p> <p> <br /> </p> <p> 串珠鐮孢霉菌(Fusarium moniliforme)CGMCC 0536的D-泛解酸內酯水解酶具有高度的立體選擇性,可立體專一性拆分DL-泛解酸內酯,生成D-泛解酸. </p></p><br /><p>文章來源地址: 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