大量供應(yīng)泛解酸內(nèi)酯銷售廠上海臨辰供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="將D-泛解酸內(nèi)酯水解酶cDNA結(jié)構(gòu)基因分別插入到大腸桿菌胞內(nèi)表達(dá)載體pTrc99a和分泌表達(dá)載體pET20b中,構(gòu)建重組質(zhì)粒pTrc99a-LAC和pET20b-LAC.將重組載體pTrc99a-LAC轉(zhuǎn)化大腸桿菌JM109.重組菌在IPT"><meta name="keywords" content="大量供應(yīng)泛解酸內(nèi)酯銷售廠"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" 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class="leftbox clearfix"><h1>大量供應(yīng)泛解酸內(nèi)酯銷售廠上海臨辰供應(yīng)</h1><div id="rmp8z3l" class="proBig"><div id="u8dkwka" class="bigimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289509208985/415071.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="87ghdw3" class="smallimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289509208985/415071.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="w9if2ab" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>大量供應(yīng)泛解酸內(nèi)酯銷售廠</p><p><span>***更新：</span>2020-06-20 02:33:55</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="m9egnbc" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="vmyz3fb" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="cj2n3pr" class="box"><p><p style="text-indent:25px">將D-泛解酸內(nèi)酯水解酶cDNA結(jié)構(gòu)基因分別插入到大腸桿菌胞內(nèi)表達(dá)載體pTrc99a和分泌表達(dá)載體pET20b中,構(gòu)建重組質(zhì)粒pTrc99a-LAC和pET20b-LAC.將重組載體pTrc99a-LAC轉(zhuǎn)化大腸桿菌JM109.重組菌在IPTG誘導(dǎo)下表達(dá)出有活性的重組酶,經(jīng)SDS-PAGE檢測,重組大腸桿菌有分子量約為40 kD的蛋白表達(dá)帶,重組酶活力平均為39 U/克濕細(xì)胞.將分泌重組載體pET20b-LAC轉(zhuǎn)化大腸桿菌BLR(DE3)pLysS，大量供應(yīng)泛解酸內(nèi)酯銷售廠,利用IPTG對重組菌進(jìn)行誘導(dǎo)，大量供應(yīng)泛解酸內(nèi)酯銷售廠，大量供應(yīng)泛解酸內(nèi)酯銷售廠,D-泛解酸內(nèi)酯水解酶被分泌表達(dá)至大腸桿菌的周質(zhì)空間,信號肽被正確識別切下后,獲得活性的D-泛解酸內(nèi)酯。D-泛解酸內(nèi)酯</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="大量供應(yīng)泛解酸內(nèi)酯銷售廠" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289509208985/415071.png"></div><p> D-泛解酸內(nèi)酯包裝:25kg紙板桶。 </p> <p> <br /> </p> <p> 性狀：白色或類白色結(jié)晶性粉末，本品溶解性：易溶于水，溶于乙醇、***、氯仿。 </p> <p> <br /> </p> <p> 合成維生素B5即D-泛酸鈣主要原料，目前采用化學(xué)合成DL-泛解酸內(nèi)酯，再經(jīng)過生物拆分得到。 </p> <p> <br /> </p> <p> D-泛解酸內(nèi)酯是一種重要的原料藥中間體。 </p> <p> <br /> </p> <p> D-泛解酸內(nèi)酯主要用于D-泛酸鈣和D-泛醇的合成。 </p> <p> <br /> </p> <p> D?泛解酸內(nèi)酯的制備方法。 </p> <p> <br /> </p> <p> 制備方法包括以下步驟：(1)將纈氨酸在過氧化氫酶和α?酮異戊酸還原酶的作用下，進(jìn)行酶轉(zhuǎn)化得到α?酮異戊酸； </p> <p> <br /> </p> <p> (2)將所述α?酮異戊酸與四氫葉酸在羥甲基轉(zhuǎn)移酶和氯化鎂的作用下，反應(yīng)制備酮泛解酸； </p> <p> <br /> </p> <p> (3)將所述酮泛解酸在α?酮異戊酸還原酶的作用下，進(jìn)行酶轉(zhuǎn)化得到泛解酸； </p> <p> <br /> </p> <p> (4)將所述泛解酸制備得到泛解酸內(nèi)酯。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="大量供應(yīng)泛解酸內(nèi)酯銷售廠" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336133632812/8293857.png"></div><p> 化還原酶催化不對稱還原制備D-泛解酸內(nèi)酯氧化還原酶不對稱還原酮泛解酸內(nèi)酯的方法。用L-泛解酸內(nèi)酯脫氫酶將L-泛解酸內(nèi)酯氧化成酮泛解酸內(nèi)酯，再利用酮泛解酸內(nèi)酯還原酶將其不對稱還原成D-泛解酸內(nèi)酯。該方法也可將外消旋的泛解酸內(nèi)酯作為起始原料，經(jīng)過氧化還原反應(yīng)后得到D-泛解酸內(nèi)酯，過程簡單，不需要化學(xué)法中的再消旋化反應(yīng)。 </p> <p> <p> 制備方法包括以下步驟：(1)將纈氨酸在過氧化氫酶和α?酮異戊酸還原酶的作用下，進(jìn)行酶轉(zhuǎn)化得到α?酮異戊酸； </p> <p> <br /> </p> <p> (2)將所述α?酮異戊酸與四氫葉酸在羥甲基轉(zhuǎn)移酶和氯化鎂的作用下，反應(yīng)制備酮泛解酸； </p> <p> <br /> </p> <p> (3)將所述酮泛解酸在α?酮異戊酸還原酶的作用下，進(jìn)行酶轉(zhuǎn)化得到泛解酸； </p> <p> <br /> </p> <p> (4)將所述泛解酸制備得到泛解酸內(nèi)酯。 </p> </p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/320970.html</p></div></div></div></div></div><div id="7aa3z4i" class="page-right twoColRt"><div 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