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>瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="rqsi0cj" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="xc332pm" class="page-proshow02"><p class="title">詳細說明</p><div id="gas2nf3" class="box"><p><p style="text-indent:25px">將D-泛解酸內酯水解酶cDNA結構基因分別插入到大腸桿菌胞內表達載體pTrc99a和分泌表達載體pET20b中,構建重組質粒pTrc99a-LAC和pET20b-LAC.將重組載體pTrc99a-LAC轉化大腸桿菌JM109.重組菌在IPTG誘導下表達出有活性的重組酶，高純泛解酸內酯有哪些品牌,經(jīng)SDS-PAGE檢測,重組大腸桿菌有分子量約為40 kD的蛋白表達帶,重組酶活力平均為39 U/克濕細胞.將分泌重組載體pET20b-LAC轉化大腸桿菌BLR(DE3)pLysS，高純泛解酸內酯有哪些品牌,利用IPTG對重組菌進行誘導,D-泛解酸內酯水解酶被分泌表達至大腸桿菌的周質空間，高純泛解酸內酯有哪些品牌,信號肽被正確識別切下后,獲得活性的D-泛解酸內酯。D-泛解酸內酯</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="高純泛解酸內酯有哪些品牌" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336133632812/8293857.png"></div><p> D-泛酸在生物體內的代謝途徑已經(jīng)確定，即經(jīng)α-酮異戊酸，利用酮泛解酸羥甲基轉移酶生成酮泛解酸，再利用酮泛解酸還原酶形成泛解酸，***與β-丙氨酸形成D-泛酸。目前，國內對維生素B5 的年需求量達2 000 t 以上，而全球年需求量達10 000 t以上 </p> <p> 化學法合成D-泛解酸內酯，從低廉原料出發(fā)經(jīng)多步反應合成，工藝成熟，但總體存在化學條件 </p> <p> 嚴苛、酸堿用量大和能耗較高等問題。生物技術的發(fā)展為生物酶法合成D-泛解酸內酯提供了充分的可行性。目前工業(yè)化合成D-泛解酸內酯采用的是化學法與水解酶拆分法相結合的技術路線。 </p> <p> <br /> </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="高純泛解酸內酯有哪些品牌" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289746210938/9268872.png"></div><p style="text-indent:25px">D-泛解酸內酯?，F(xiàn)有技術中還有在培養(yǎng)基中培養(yǎng)尖孢鐮孢菌，在培養(yǎng)后的尖孢鐮孢菌與底物混合，水解得到D-泛解酸，內酯化獲得D-泛解酸內酯，但是本方法采用發(fā)酵制備D-泛解酸內酯的過程能耗較高，且產(chǎn)率低，工業(yè)成本較高?，F(xiàn)有技術中還有以水和甲苯為溶劑體系，通過添加D-泛解酸內酯作為晶種，誘導結晶獲得D-泛解酸內酯的方法，但是本方法在生產(chǎn)過程中采用了甲苯，生產(chǎn)過程VOC排放較高，且通過誘導結晶獲得的一次收率較低，產(chǎn)品比旋度較低。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/341261.html</p></div></div></div></div></div><div id="yszj4mo" class="page-right twoColRt"><div id="crto9hz" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="f7mt4ph" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強信息推薦自業(yè)物資供應" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強信息推薦自業(yè)物資供應</p></a></div><div id="pmel4mo" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/846569.html"><div><img 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