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href="http://www.cdcfah.com/cp/second-_yyby/">醫(yī)藥健康</a>?<a href="http://www.cdcfah.com/cp/third-yyby_zyfl/">制藥輔料</a>?**泛解酸內(nèi)酯批發(fā)廠上海臨辰供應(yīng)</p><div id="3vlzdtj" class="leftbox clearfix"><h1>**泛解酸內(nèi)酯批發(fā)廠上海臨辰供應(yīng)</h1><div id="xbt3p7h" class="proBig"><div id="x3zjp3l" class="bigimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289561562500/2636284.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="lbhv3tx" class="smallimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289561562500/2636284.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="z3lzrt5" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>**泛解酸內(nèi)酯批發(fā)廠</p><p><span>***更新：</span>2020-06-22 10:28:59</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="5rxzpfj" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="dfhjbtx" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="npd5lz5" class="box"><p><p style="text-indent:25px">篩選到一株產(chǎn)D-泛解酸內(nèi)酯水解酶的串珠鐮孢霉菌(Fusarium moniliforme SW902).產(chǎn)酶條件研究表明,用甘油作碳源,蛋白胨作氮源,初始pH8.0,溫度26℃,搖瓶培養(yǎng)3 d,產(chǎn)酶量比較高.在60L和1000L發(fā)酵罐中通風(fēng)發(fā)酵45～47h,產(chǎn)酶量為6～8g干菌體/L,D-泛解酸內(nèi)酯水解酶酶活力達(dá)到0.87～0.92IU/g干菌體.該酶的**適反應(yīng)溫度為55℃，**泛解酸內(nèi)酯批發(fā)廠,**適反應(yīng)pH為7.0～7，**泛解酸內(nèi)酯批發(fā)廠.5.在酶不對稱水解泛解酸內(nèi)酯過程中，**泛解酸內(nèi)酯批發(fā)廠,對溶液加酶量5%～10%,底物濃度10%～20%,控制水解率20%～30%,水解效果比較好。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="**泛解酸內(nèi)酯批發(fā)廠" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289561562500/2636284.png"></div><p style="text-indent:25px">一種微生物新菌株內(nèi)生***HL-02 (PlectospHaereella cucumerina HL-2)及其在微生物轉(zhuǎn)化制備D-泛解酸內(nèi)酯中的應(yīng)用，所述的應(yīng)用以DL-泛解酸內(nèi)酯為底物，以內(nèi)生*** HL-2發(fā)酵所得菌液或菌液制備的初酶液為酶源，20～45℃下，在含有氯化鈣的Tris-HCl緩沖溶液的反應(yīng)體系中進(jìn)行水解反應(yīng)得水解液，取水解液用乙酸乙酯進(jìn)行酯化反應(yīng)，反應(yīng)液經(jīng)后處理制得D-泛解酸內(nèi) 酯；本發(fā)明提供了一株新的可產(chǎn)D-泛解酸內(nèi)酯水解酶的微生物菌種內(nèi)生***HL-2，該菌種應(yīng)用于拆分DL-泛解酸內(nèi)酯，立體選擇性好，反應(yīng)條件溫和。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="**泛解酸內(nèi)酯批發(fā)廠" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289509208985/415071.png"></div><p> 該酶已經(jīng)成功的應(yīng)用于工業(yè)化,取得了很好的綜合效益.為了提高D-泛解酸內(nèi)酯水解酶的酶活力及在低pH條件下的穩(wěn)定性,我們在基因水平上對酶進(jìn)行了研究. 利用RT-PCR方法,擴(kuò)增得到了編碼源于串珠鐮孢霉菌(Fusarium moniliforme)CGMCC </p> <p> 0536的D-泛解酸內(nèi)酯水解酶cDNA,對其結(jié)構(gòu)進(jìn)行分析,根據(jù)分析結(jié)果設(shè)計了一對特異引物,成功擴(kuò)增到了編碼D-泛解酸內(nèi)酯水解酶cDNA的結(jié)構(gòu)基因,結(jié)構(gòu)基因同pMD18-T載體連接后,進(jìn)行測序.D-泛解酸內(nèi)酯水解酶cDNA結(jié)構(gòu)基因序列已在Genebank數(shù)據(jù)庫申請登記,登記號為AY728018. </p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/348731.html</p></div></div></div></div></div><div id="jpfjnrh" class="page-right twoColRt"><div id="ndrjnpv" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="lndf5z5" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" 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