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class="button">聯(lián)系我們</a></div></div><div id="7b7lhzt" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="xpbl79p" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="rdpt9hj" class="box"><p><p style="text-indent:25px">篩選到一株產(chǎn)D-泛解酸內(nèi)酯水解酶的串珠鐮孢霉菌(Fusarium moniliforme SW902).產(chǎn)酶條件研究表明,用甘油作碳源,蛋白胨作氮源,初始pH8.0,溫度26℃,搖瓶培養(yǎng)3 d,產(chǎn)酶量比較高.在60L和1000L發(fā)酵罐中通風(fēng)發(fā)酵45～47h,產(chǎn)酶量為6～8g干菌體/L,D-泛解酸內(nèi)酯水解酶酶活力達(dá)到0.87～0.92IU/g干菌體.該酶的**適反應(yīng)溫度為55℃,**適反應(yīng)pH為7.0～7.5，穩(wěn)定供應(yīng)泛解酸內(nèi)酯產(chǎn)品介紹.在酶不對稱水解泛解酸內(nèi)酯過程中,對溶液加酶量5%～10%，穩(wěn)定供應(yīng)泛解酸內(nèi)酯產(chǎn)品介紹,底物濃度10%～20%，穩(wěn)定供應(yīng)泛解酸內(nèi)酯產(chǎn)品介紹,控制水解率20%～30%,水解效果比較好。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="穩(wěn)定供應(yīng)泛解酸內(nèi)酯產(chǎn)品介紹" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278338760781250/415124.png"></div><p style="text-indent:25px">一種微生物新菌株內(nèi)生***HL-02 (PlectospHaereella cucumerina HL-2)及其在微生物轉(zhuǎn)化制備D-泛解酸內(nèi)酯中的應(yīng)用，所述的應(yīng)用以DL-泛解酸內(nèi)酯為底物，以內(nèi)生*** HL-2發(fā)酵所得菌液或菌液制備的初酶液為酶源，20～45℃下，在含有氯化鈣的Tris-HCl緩沖溶液的反應(yīng)體系中進(jìn)行水解反應(yīng)得水解液，取水解液用乙酸乙酯進(jìn)行酯化反應(yīng)，反應(yīng)液經(jīng)后處理制得D-泛解酸內(nèi) 酯；本發(fā)明提供了一株新的可產(chǎn)D-泛解酸內(nèi)酯水解酶的微生物菌種內(nèi)生***HL-2，該菌種應(yīng)用于拆分DL-泛解酸內(nèi)酯，立體選擇性好，反應(yīng)條件溫和。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="穩(wěn)定供應(yīng)泛解酸內(nèi)酯產(chǎn)品介紹" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289561562500/2636284.png"></div><p style="text-indent:25px">羥基腈裂合酶催化不對稱合成D-泛解酸內(nèi)酯前體(R)-氰醇(R)-氰醇是D-泛解酸內(nèi)酯的前體物質(zhì)，可經(jīng)過水解生產(chǎn)D-泛解酸內(nèi)酯。利用(R)-羥基腈裂合酶催化HCN 與羥基特戊醛的羰基碳不對稱加成進(jìn)而合成(R)-氰醇。大多數(shù)參與醇腈化反應(yīng)的酶主要存在于高等植物中，例如巴旦杏、蘋果、薔薇、橡膠樹、亞麻類等，在植物體內(nèi)羥基腈裂解酶催化形成氫腈酸從而抵御外來物種的入侵。2007 年荷蘭DSM 公司從巴旦杏中提取分離的(R)-羥基腈裂合酶能選擇性合成(R)-氰醇，并構(gòu)建了粟酒裂殖酵母工程菌。2014 年，DSM 公司將來自薔薇科的(R)-羥基腈裂合酶基因構(gòu)建于pJET 重組質(zhì)粒，并導(dǎo)入巴斯德酵母中表達(dá)，確定了該重組酶**適條件范圍為15~20 ℃以及pH 2.5~3.0。在15 ℃和pH 2.5 下醇腈化反應(yīng)2 d，反應(yīng)收率達(dá)到93%，轉(zhuǎn)化率達(dá)**，產(chǎn)物光學(xué)純度達(dá)92%。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/349924.html</p></div></div></div></div></div><div id="d7vb7bf" 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