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id="keqarrt" class="proBig"><div id="wqifh2e" class="bigimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289746210938/9268872.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="t3d3qnk" class="smallimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289746210938/9268872.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="cgyac78" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>D泛解酸內(nèi)酯高質(zhì)量的選擇</p><p><span>***更新：</span>2020-06-23 13:26:12</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ui7i2zg" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="odqxeli" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="s83zoln" class="box"><p><p> D-泛酸鈣的制備方法主要有兩種：1)先化學(xué)合成DL-泛酸鈣，通過拆分DL-泛酸鈣而制得D-泛酸鈣。2)通過化學(xué)法或生物法拆分關(guān)鍵中間體DL-泛解酸內(nèi)酯得到D-泛解酸內(nèi)酯，再由D-泛解酸內(nèi)酯與β--氨基丙酸反應(yīng)合成D-泛酸鈣，其中生物法拆分的成本比化學(xué)法拆分的成本低15%，D泛解酸內(nèi)酯高質(zhì)量的選擇。 </p> <p> 國外D-泛酸鈣生產(chǎn)公司主要有日本制藥精細(xì)化學(xué)品公司、德國BASF公司，D泛解酸內(nèi)酯高質(zhì)量的選擇，D泛解酸內(nèi)酯高質(zhì)量的選擇、荷蘭DSM公司等。BASF、DSM均采用化學(xué)法生產(chǎn)，而日本***制藥采用生物法酶拆分工藝制備中間體D-泛解酸內(nèi)酯，這也是目前**技術(shù)的工藝。國內(nèi)原只有鑫富藥業(yè)一家有生物法酶拆分工藝，但現(xiàn)在山東新發(fā)也采用了這一工藝。 </p> <p> <br /> </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="D泛解酸內(nèi)酯高質(zhì)量的選擇" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289746210938/9268872.png"></div><p style="text-indent:25px">選擇6種吸附樹脂和離子交換樹脂對D-泛解酸內(nèi)酯水解酶進(jìn)行固定化,篩選出了固定化效果較好的大孔弱堿性丙烯酸系陰離子交換樹脂D-380為載體,用先吸附后交聯(lián)的方法固定化。通過實驗對固定化條件進(jìn)行了優(yōu)化,得出比較好的固定化條件為:加酶量6 U/g樹脂、吸附pH 7.5、吸附時間4 h、吸附溫度30℃、交聯(lián)劑戊二醛終濃度0.1%、交聯(lián)時間2 h。實驗表明在此條件下制得的固定化酶有很好的穩(wěn)定性:固定化酶在連續(xù)20次的底物水解反應(yīng)后,剩余酶活達(dá)到71%。當(dāng)溫度達(dá)到80℃時游離酶幾乎失去酶活,而固定化酶剩余酶活為60%以上。游離酶的pH穩(wěn)定性范圍為pH 7～8,而固定化酶為pH 6.5～8.5。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="D泛解酸內(nèi)酯高質(zhì)量的選擇" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336263828125/6823745.png"></div><p> 該酶已經(jīng)成功的應(yīng)用于工業(yè)化,取得了很好的綜合效益.為了提高D-泛解酸內(nèi)酯水解酶的酶活力及在低pH條件下的穩(wěn)定性,我們在基因水平上對酶進(jìn)行了研究. 利用RT-PCR方法,擴增得到了編碼源于串珠鐮孢霉菌(Fusarium moniliforme)CGMCC </p> <p> 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