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id="qpbiulh" class="smallimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289561562500/2636284.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="dh3yul8" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關鍵詞：</span>食品級泛解酸內(nèi)酯批發(fā)價,泛解酸內(nèi)酯</p><p><span>***更新：</span>2020-06-30 17:29:31</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="2f3kry3" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海臨辰醫(yī)藥科技有限公司</a></p><p><span>聯(lián)系人：</span>葉海岑</p><p><span>郵箱: </span>info@linchenpharm.com</p><p><span>電話: </span>13761181955</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-31606595</p><p><span>地址: </span>辰花路5088弄221號</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="ra2qs82" class="page-proshow02"><p class="title">詳細說明</p><div id="b3a8g2b" class="box"><p><p style="text-indent:25px">將D-泛解酸內(nèi)酯水解酶cDNA結構基因分別插入到大腸桿菌胞內(nèi)表達載體pTrc99a和分泌表達載體pET20b中,構建重組質粒pTrc99a-LAC和pET20b-LAC.將重組載體pTrc99a-LAC轉化大腸桿菌JM109.重組菌在IPTG誘導下表達出有活性的重組酶,經(jīng)SDS-PAGE檢測,重組大腸桿菌有分子量約為40 kD的蛋白表達帶,重組酶活力平均為39 U/克濕細胞，食品級泛解酸內(nèi)酯批發(fā)價.將分泌重組載體pET20b-LAC轉化大腸桿菌BLR(DE3)pLysS,利用IPTG對重組菌進行誘導，食品級泛解酸內(nèi)酯批發(fā)價，食品級泛解酸內(nèi)酯批發(fā)價,D-泛解酸內(nèi)酯水解酶被分泌表達至大腸桿菌的周質空間,信號肽被正確識別切下后,獲得活性的D-泛解酸內(nèi)酯。D-泛解酸內(nèi)酯中文別名:D-泛酰內(nèi)酯。食品級泛解酸內(nèi)酯批發(fā)價</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="食品級泛解酸內(nèi)酯批發(fā)價,泛解酸內(nèi)酯" src="https://tyunfile.71360.com/WaterMark/UploadFile//637280289561562500/2636284.png"></div><p style="text-indent:25px">“微生物酶法拆分制備D－泛解酸內(nèi)酯及用于生產(chǎn)D-泛酸鈣與D-泛醇”項目選育了一株能高產(chǎn)立體專一性D-泛解酸內(nèi)酯水解酶、不利用不降解泛解酸內(nèi)酯或泛解酸的微生物菌株串珠鐮孢霉，建立了國際**的霉菌交聯(lián)原位固定化方法，酶轉化時間短到3～5小時，效率高，反復分批酶轉化可達180次以上，所得到的酶水解產(chǎn)物光學純度達到99％e．e．以上，因此具有較好的技術經(jīng)濟競爭力。該項目采用的D-泛酸鈣生產(chǎn)能力微生物酶催化拆分方法，與傳統(tǒng)化學拆分方法相比，以生產(chǎn)D-泛醇為例，原材料消耗減少69．2％，廢液、廢渣排放分別減少65．5％、43．8％，能耗減少12．7％，生產(chǎn)成本降低了26．5％，同時，生物法改善了操作環(huán)境，提高了產(chǎn)品品質與產(chǎn)品安全性，提高了D-泛酸生產(chǎn)的整體技術水平，達到了用高新生物技術改造傳統(tǒng)化學工業(yè)的目的。藥用級泛解酸內(nèi)酯發(fā)酵D-泛解酸內(nèi)酯是一種混旋泛酸鈣的中間體。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="食品級泛解酸內(nèi)酯批發(fā)價,泛解酸內(nèi)酯" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336219531250/8019769.png"></div><p> 以水和甲苯為溶劑體系，通過添加D-泛解酸內(nèi)酯作為晶種，誘導結晶獲得D-泛解酸內(nèi)酯的方法。 </p> <p> <br /> </p> <p> 但本法在生產(chǎn)過程中用了甲苯，生產(chǎn)過程VOC排放較高，且通過誘導結晶獲得的一次收率較低，產(chǎn)品比旋度較低。 </p> <p> <br /> </p> <p> D-泛解酸內(nèi)酯的工業(yè)制備是由DL-泛解酸內(nèi)酯拆分而來的。 </p> <p> <br /> </p> <p> 作為一種重要的醫(yī)藥中間體，主要用于合成維生素類藥D-泛醇及神經(jīng)營養(yǎng)藥D-泛酸鈣。 </p> <p> <br /> </p> <p> D-泛酸鈣是維生素B5，作為重要的營養(yǎng)成分，***用于飼料添加劑、食品添加劑、醫(yī)藥等領域； </p> <p> <br /> </p> <p> D-泛醇是維生素原B5，***用于護發(fā)用品和化妝品中。 </p> <p> <br /> </p><p style="text-indent:25px">選擇6種吸附樹脂和離子交換樹脂對D-泛解酸內(nèi)酯水解酶進行固定化,篩選出了固定化效果較好的大孔弱堿性丙烯酸系陰離子交換樹脂D-380為載體,用先吸附后交聯(lián)的方法固定化。通過實驗對固定化條件進行了優(yōu)化,得出比較好的固定化條件為:加酶量6 U/g樹脂、吸附pH 7.5、吸附時間4 h、吸附溫度30℃、交聯(lián)劑戊二醛終濃度0.1%、交聯(lián)時間2 h。實驗表明在此條件下制得的固定化酶有很好的穩(wěn)定性:固定化酶在連續(xù)20次的底物水解反應后,剩余酶活達到71%。當溫度達到80℃時游離酶幾乎失去酶活,而固定化酶剩余酶活為60%以上。游離酶的pH穩(wěn)定性范圍為pH 7～8,而固定化酶為pH 6.5～8.5。D-泛解酸內(nèi)酯是合成D-泛酸的重要中間體。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="食品級泛解酸內(nèi)酯批發(fā)價,泛解酸內(nèi)酯" src="https://tyunfile.71360.com/WaterMark/UploadFile/shlinchen/637278336263828125/6823745.png"></div><p style="text-indent:25px">醇脫氫酶參與的化學酶法合成D-泛解酸內(nèi)酯與D- 泛解酸內(nèi)酯的工業(yè)合成類似，從縮醛反應出發(fā)再經(jīng)醇脫氫酶還原， *經(jīng)過三步就合成了D- 泛解酸內(nèi)酯。這種醇脫氫酶參與的化學酶法合成過程由德國比勒費爾德大學HEIDLINDEMANN M 等在2015 年設計以異丁醛和乙醛酸乙酯為起始原料，以L-組氨酸作為有機催化劑在酸性條件下發(fā)生醛醛縮合反應，得到(R)-中間體1。醇脫氫酶以異丙醇為輔底物實現(xiàn)輔酶循環(huán)，將中間體1 還原成中間體2，中間體2 無需分離直接自發(fā)環(huán)化成D-泛解酸內(nèi)酯。反應中D-泛解酸內(nèi)酯的總得率達55%，產(chǎn)物e.e.值達到95%。將所述酮泛解酸在α?酮異戊酸還原酶的作用下，進行酶轉化得到泛解酸。98泛解酸內(nèi)酯中間體</p><p style="text-indent:25px">作為腦代謝的D-泛解酸內(nèi)酯，主要用于生產(chǎn)泛酸鈣。食品級泛解酸內(nèi)酯批發(fā)價</p><p> 傳統(tǒng)的生產(chǎn)方法是化學拆分法或鈣鹽誘導結晶法?；瘜W拆分的缺點是拆分劑太貴，分離困難，步驟多，成本高，還0性和環(huán)境污染問題。鈣鹽誘導結晶法步驟多，收率低，投資大，擴大生產(chǎn)規(guī)模比較困難。另外，誘導結晶法不能用于D－泛醇等泛酸衍生物生產(chǎn)。 </p> <p> “微生物酶法拆分制備D－泛解酸內(nèi)酯及用于生產(chǎn)D－泛酸鈣與D－泛醇”項目選育了一株能高產(chǎn)立體專一性D－泛解酸內(nèi)酯水解酶、不利用不降解泛解酸內(nèi)酯或泛解酸的微生物菌株串珠鐮孢霉，建立了國際**的霉菌交聯(lián)原位固定化方法，酶轉化時間短到3～5小時，效率高，反復分批酶轉化可達180次以上，所得到的酶水解產(chǎn)物光學純度達到99％e．e．以上，因此具有較好的技術經(jīng)濟競爭力。食品級泛解酸內(nèi)酯批發(fā)價</p><p style="text-indent:25px">上海臨辰醫(yī)藥科技有限公司坐落在辰花路5088弄221號，是一家專業(yè)的Plasdone?聚維酮(PVP)，Polyplasdone?交聯(lián)聚維酮(PVPP)，Plasdone?共聚維酮（PVP/VA)，Benecel?羥丙甲纖維素(HPMC)，Klucel?羥丙纖維素(HPC) 硬脂富馬酸鈉，羧甲基淀粉鈉，交聯(lián)羧甲基纖維素鈉，微晶纖維素，磷酸氫鈣（無水/二水）USP-NF,Ph.Eur,JP" D-(+)-半乳糖（植物）蔗糖，異麥芽糖，異麥芽酮糖醇，三氯蔗糖，環(huán)氨酸鈉（甜蜜素)，赤蘚糖醇丁香酚，丁香油，蓖麻油，芝麻油，橄欖油，中鏈甘油三酸酯，乙酰單酸甘油乙酯，蔗糖脂肪酸酯聚乙二醇3350/1000/4000/6000/8000，白陶土，液體石蠟，輕質液體石蠟，（干燥）氫氧化鋁凝膠，薄荷腦，合成鋁碳酸鎂，合成硅酸鋁，氧化鎂，氫氧化鎂，甘羥鋁，甘氨酸鋁，磷酸鈣（二水/無水），三硅酸鎂，滑石粉，BHA,BHT,多庫酯鈉，組氨酸，鹽酸組氨酸，精氨酸，鹽酸精氨酸，絲氨酸，半胱氨酸，丙氨酸， Pfanstiehl海藻糖（二水），蔗糖（甘蔗/甜菜），甘露醇，精氨酸，琥珀酸二鈉（無水），氨丁三醇 (含低內(nèi)*素），活性炭（供注射）色素系列黃/紅氧化鐵，檸檬黃色淀，公司。唯才是舉，唯能是用：擁有優(yōu)秀人才5~10人和，是實現(xiàn)企業(yè)戰(zhàn)略目標的基礎，是企業(yè)持續(xù)發(fā)展的動力。公司以誠信為本，業(yè)務領域涵蓋[ "泛解酸內(nèi)酯", "有機可可粉有機果粉", "阿拉伯膠瓜爾膠 ", "BIS TRIS " ]，我們本著對客戶負責，對員工負責，更是對公司發(fā)展負責的態(tài)度，爭取做到讓每位客戶滿意。公司力求給客戶提供***質量服務，我們相信誠實正直、開拓進取地為公司發(fā)展做正確的事情，將為公司和個人帶來共同的利益和進步。經(jīng)過幾年的發(fā)展，已成為[ "泛解酸內(nèi)酯", "有機可可粉有機果粉", "阿拉伯膠瓜爾膠 ", "BIS TRIS " ]行業(yè)**企業(yè)。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/449784.html</p></div></div></div></div></div><div id="lfry8ds" class="page-right twoColRt"><div id="ixelsoo" class="box01 margin10 clearfix"><h3><a 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